大白菜与甘蓝种间BC<sub>1</sub>的减数分裂观测及其性状鉴定

doi:10.7668/hbnxb.2010.S1.020

华北农学报 ›› 2010, Vol. 25 ›› Issue (S1): 86-89. doi: 10.7668/hbnxb.2010.S1.020

所属专题： 白菜；

大白菜与甘蓝种间BC₁的减数分裂观测及其性状鉴定

孟雅宁, 陈雪平, 刘畅, 申书兴

河北农业大学园艺学院, 河北保定 071000

收稿日期:2010-06-10 出版日期:2010-12-30
通讯作者: 申书兴(1964-),男,河北滦南人,教授,博士,主要从事蔬菜生物技术和遗传育种研究
作者简介:孟雅宁(1984-),女,河北石家庄人,硕士,主要从事蔬菜生物技术和遗传育种研究
基金资助:
河北省自然科学基金资助项目(C2005000222);河北农业大学科技将帅计划

Meiosis Observation and Behaviour Evaluation of Interspecific BC1 Derived from Chinese Cabbage and Cabbage

MENG Ya-ning, CHEN Xue-ping, LIU Chang, SHEN Shu-xing

College of Horticulture,Hebei Agricultural University, Baoding 071000

Received:2010-06-10 Published:2010-12-30

摘要/Abstract

摘要： 以回交父本二倍体大白菜(代号为85-1,2n=20)为对照,对异源三倍体(AAC)与二倍体大白菜(AA)回交的BC₁(代号为a2,2n=22)进行花粉孢母细胞减数分裂观察,并对其生殖时期田间性状和花粉特性进行了观测。结果表明:花粉孢母细胞减数分裂复杂,终变期观察到8Ⅱ+2Ⅲ、10Ⅱ+2Ⅰ、9Ⅱ+1Ⅲ+1Ⅰ等多种联会方式;中期I、II出现单个或多个游离染色体;后期I、II出现落后染色体或丢失染色体的频率很高,导致多种方式的分离,出现了较少的、利用价值较高的11条染色体的配子。此外,田间观察证明,与85-1相比较,a2生长势弱,花粉量和生活力均较低,叶片、花和花蕾都较小,抽薹和开花较早。上述结果为大白菜杂交育种的后代种植群体设置和调节花期提供了理论依据。

关键词: 大白菜, 回交, 减数分裂, 染色体

Abstract: The pollen mother cells(PMC) meiosis of BC₁ derived from allotriploid(AAC) and diploid Chinese cabbage(AA) were studied,and both farm characteristics and pollen character of BC1 were evaluated at reproductive stage,using diploid Chinese cabbage(codenamed 85-1,2n = 20) as a control. The results showed that the chromosome division during PMC meiosis appeared complicated. Multi-synapsis types(8Ⅱ + 2Ⅲ,10Ⅱ + 2Ⅰ,9Ⅱ + 1 Ⅲ + 1Ⅰ) were observed at diakinesis stage. Single or a few isolated free chromosomes were funded during metaphaseⅠ andⅡ. The frequency of chromosome lagging or losing was very high during anaphaseⅠandⅡ. The results mentioned above may be the main reasons which caused a variety of ways of chromosomes separation. There were a few valuable gametes with 11 chromosomes appeared. In addition,field observation showed that both the growth vigor and pollen quantity or viability of a2 were weaker or lower than that of 85-1. A2 had smaller leaves,flowers and flower buds,and had earlier bolting and flowering trait,comparing with 85-1. All the results mentioned above provided a theoretical basis for progeny planting population setting up and regulating flowering date in cross-breeding of Chinese cabbage.

Key words: Chinese cabbage, Backcross, Meiosis, Chromosome

中图分类号:

S634.1

孟雅宁, 陈雪平, 刘畅, 申书兴. 大白菜与甘蓝种间BC₁的减数分裂观测及其性状鉴定[J]. 华北农学报, 2010, 25(S1): 86-89. doi: 10.7668/hbnxb.2010.S1.020.

MENG Ya-ning, CHEN Xue-ping, LIU Chang, SHEN Shu-xing. Meiosis Observation and Behaviour Evaluation of Interspecific BC1 Derived from Chinese Cabbage and Cabbage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(S1): 86-89. doi: 10.7668/hbnxb.2010.S1.020.

http://www.hbnxb.net/CN/Y2010/V25/IS1/86

参考文献

[1] Jiang J,Friebe B,Gill B S.Recent advance in alien genes transfer in wheat[J].Euphytica,1994,73:199-212.
[2] Chen B Y,Heneen W K,Jsson R.Resynthesis of Brassica napus L.through interspecific hybridization between B.alboglabra Bailey and B.campestris L.With special emphasis on seed color[J].Plant breeding,1988,101:52-59.
[3] 山东农业大学.蔬菜栽培各论[M].北京:中国农业出版社,2000:78-93.
[4] Peterka H,Budahn H,Schrader O,et al.Transfer of resistance against the beet cyst nematode from radish(Raphanus sati-vus)to rape(Brassica napus)by monosomic chromosome addition[J].Theoretical and Applied Genetics,2004,109:30-41.
[5] 王玉霞,王颖,李芳东,等.不同种仁率山楂品种花粉量及花粉活力的测定[J].山东农业科学,2008,2(35):8-10.
[6] McGrath J M,Quiros C F.Inheritance of isozyme and RFLP makers in Brassica campestris and comparison with B.oleracea[J].Theoretical and Applied Genetics,1991,82:668-673.
[7] Hu J,Quiros C F.Application of molecular markers and cytogenetic stocks to Brassica genetics,breeding and evolution[J].Act orticulturae,1996,407:79-85.
[8] Cheung W Y,champagne G,hubert N,et al.Comparison of the genetic maps of Brassica napus and Brassica oleraca[J].Theoretical and Applied Gnetics,1997,94:569-582.
[9] 潘大仁.油菜(Bnapus)属种间杂种后代的遗传研究[J].农业生物技术学报.1999,7(2):141-146.
[10] 黄邦全,刘幼琪,吴文华,等.Ogura CMS 紫菜薹×萝卜×甘蓝型油菜杂种的获得及细胞遗传学研究[J].遗传学报,2002,29(5):467-470.
[11] 桑丹,刘畅,王娟,等.大白菜-结球甘蓝5号和8号单体异附加系的筛选和鉴定[J],2008,9(4):517-520.
[12] 李宗芸,栗茂腾,黄荣桂,等.基因组原位杂交辨别芸薹属异源四倍体AA、BB、CC基因组研究[J].中国油料作物学报,2002,24(1):10-14.
[13] 刘畅,王彦华,轩淑欣,等.大白菜-结球甘蓝4号单体异附加系的鉴定[J].农业生物技术学报,2008,16(6):990-994.
[14] 栗根义,高睦枪,杨建平.白菜-甘蓝染色体附加系的性状遗传[J].华北农学报,1996,11(2):96-100.
[15] Quiros C F,Ochoa O,Kianian S F,et al.Analysis of the Brassica oleracea genome by the generation of B.campestris oleracea chromosome addition lines:Characterization by isozymes and rDNA genes[J].Theoretical and App lied Genetics,1987,74:758-766.
[16] 顾爱侠,郑宝智,王彦华,等.附加甘蓝3号染色体的大白菜单体异附加系的获得与研究[J].园艺学报,2009,36(1):39-44.
[17] Chen B Y,Simonsen V,Lannér Herrera C,et al.A Brassica cam pestris-alboglabra addition line and its use for gene mapping,intergenomic gene transfer and generation of trisomics[J].Theoretical and Applied Genetics,1992,84:592-599.
[18] Cheng B F,HeneenW K,Chen B Y.Mitotic karyotypes of Brassica cam pestris and Brassica alboglabra and identification of the B.alboglabra chromosome in an addition line[J].Genome,1995,38:313-319.
[19] Hasterok R,Wolny E,Kulak S,et al.Molecular cytogenetic analysis of Brassica rapa-Brassica oleracea var.alboglabra monosomic addition lines[J].Theoretical and Applied Genetics,2005,111:196-205.
[20] MitchellMcGrath J,Quiros C F,Harada J J,et al.Identification of Brassica oleracea monosomic alien chromosome addition lines with molecular markers reveals extensive gene duplication[J].Molecular Genetics and Genomics,1990,223:198-204.

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大白菜与甘蓝种间BC₁的减数分裂观测及其性状鉴定

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大白菜与甘蓝种间BC1的减数分裂观测及其性状鉴定

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大白菜与甘蓝种间BC₁的减数分裂观测及其性状鉴定