摘要： 对来自江苏、云南、山东、河北等地灰飞虱来源的21个水稻条纹病毒分离物的病害特异性蛋白(sp)基因进行了遗传多样性分析研究。序列测定表明,所有分离物的sp基因大小相同,均由537个核苷酸组成,编码178个氨基酸和1个终止密码子。采用DNAStar软件进行分析,21个RSV分离物核苷酸和推导的编码蛋白氨基酸序列同源性分别为96.1%~100%和95.5%~100%。与已报道水稻来源的27个RSV分离物一起进行序列同源性比较和系统进化树分析,结果表明,RSV-sp基因核苷酸序列变异相对较大,除灰飞虱来源的或者云南来源的分离物相对独立成组外无明显的规律性。从功能上分析,其遗传多样性首先与寄主相关,从寄主角度可以划分为灰飞虱和水稻两个寄主群;其次与地缘相关,在灰飞虱寄主群中可以分成中国云南及云南以外的两个地理亚群,在水稻寄主群中可以划分为中国云南和云南以外的多个地理亚群。

关键词: 水稻条纹病毒, sp基因, 遗传多样性, 寄主群, 地理亚群

Abstract: The genetic diversity of disease-specific protein(sp)from twenty-one isolates of Rice striPe virus in Laodelphax striateUus collected from Jiangsu, Yunnan, Shandong and Hebei provinces was analyzed.Sequence analysis results showed that the cDNA include 537 bp of sp gene and encode 178 amino acids and a stop codon.By DNAStar software, the similarities of nucleotide and deduced amino acid sequences of the sp genes among these isolates were 96.1%to 100%and 95.0%to 99.4%.respectively.The nucleotide sequences obtained in the study were compared with previously reported sequences of other 27 isolates in rice.Results demonstrated that the nucleotide of RSV-sp gene had larger molecular variation, and had no obviously regularity except that the isolates from Laodelphax striateUus and Yunnan belonged to an independent group.Functional analysis revealed that the genetic diversity of RSV-sp gene was related to host first which was divided into two hosts colonies：Laodelphax striatellus and rice.In addition, it was correlated with area secodenly which was divided into two geographical colonies in Laodelphax striatellus colony and many geographical colonies in rice colony：Yunnan in China, the rests without Yunnan in China.

Key words: Rice stripe virus, Disease—specific protein gene, Genetic diversity, Hosts colony, Geographical colony

中图分类号: 

• S435.111.4