摘要: 为了从普通番茄中筛选耐贮的优良育种材料,以番茄(Lycopersicon esculentum)07g-31(红果)、07g-32(粉果、F1)、07g-33(粉果、父本)、07g-34(粉果、母本)为试验材料,研究耐贮性不同的番茄果实成熟软化过程中呼吸、乙烯生理、硬度、多聚半乳糖醛酸酶及果实细胞壁超微结构的变化差异.结果表明:在耐贮性方面表现出明显优势的07g-31、07g-33果实成熟软化过程中,呼吸峰来临时间晚、强度弱,乙烯释放量低,ACC含量、ACC合成酶和ACC氧化酶的活性偏低,硬度下降较慢,多聚半乳糖醛酸酶活性较低,细胞壁及胞间层分裂缓慢、程度轻,07g-34的表现恰好相反,而07g-33、07g-34的杂交一代07g-32在以上各方面的表现介于双亲之间.
关键词:
番茄,
呼吸,
乙烯,
多聚半乳糖醛酸酶,
超微结构,
耐贮性
Abstract: Tomato-(Lycopersicon-esculentum)-07g-31(Red) ,07g-32(Pink,F1) ,07g-33(Pink,male parent) ,07g-34(Pink,female parent) was used to investigate the changes of respiration and ethylene physiology,firmness,polyga-lacturonase and cell wall ultrastructure of tomato fruits with different storability in the process of ripening and softening. The result indicated that the storability of 07g-31,07g-33 was better,their climacteric peak delay with lower intensity and less ethylene production,ACC content,ACC synthase and ACC oxidase. They intenerate more slowly,the activity of polygalacturonase were lower,the cell wall and middle lamella break up more slowly and the degree were lesser,07g-34 behaves just the reverse,the exhibitions of F1 hybrid 07g-32 were between 07g-33(male parent) and 07g-34 (female parent) in above aspects.
Key words:
Tomato,
Respiration,
Ethylene,
PG,
Ultrastructure,
Storability
中图分类号:
冯锡刚, 王孝宣, 国艳梅, 杜永臣, 胡鸿. 耐贮性不同番茄果实成熟过程中生理特性差异的研究[J]. 华北农学报, 2010, 25(5): 121-126. doi: 10.7668/hbnxb.2010.05.025.
FENG Xigang, WANG Xiaoxuan, GUO Yanmei, DU Yongchen, HU Hong. Research of Physiological Difference of Tomato Fruits with Different Storability during Ripening[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(5): 121-126. doi: 10.7668/hbnxb.2010.05.025.