摘要： 旨在研究莱芜猪和杜洛克猪间肌肉H-FABP基因mRNA表达差异,同时分析H-FABP基因mRNA表达量与肌内脂肪和脂肪酸含量的相关性,探索莱芜猪肌内脂肪沉积的分子机理.试验选择100 kg体重莱芜猪10头、杜洛克7头,采用荧光定量RT-PCR法测定H-FABP基因mRNA表达丰度,并测定肌内脂肪和脂肪酸含量.结果表明,莱芜猪背最长肌H-FABP基因mRNA的表达量比杜洛克猪高36.16%.莱芜猪和杜洛克猪肌肉H-FABP基因mRNA的表达量与肌内脂肪含量相关显著,H-FABP基因mRNA的表达量和肌内脂肪含量与饱和脂肪酸、饱和+单不饱和脂肪酸含量相关显著,莱芜猪H-FABP基因mRNA的表达量和肌内脂肪含量与多不饱和脂肪酸含量相关显著,与脂肪酸总量相关不显著,但是杜洛克猪则与此相反.莱芜猪和杜洛克猪肌肉H-FABP基因mRNA表达量与肌内脂肪和脂肪酸组成相关显著,该基因可作为莱芜猪肌内脂肪选育的候选基因.

关键词: 莱芜猪, 荧光定量RT-PCR, H-FABP基因mRNA表达量, 肌内脂肪, 脂肪酸

Abstract: This experiment was conducted to study the differential expression of H-FABP gene between Laiwu pigs and Duroc，and analysis correlation of H-FABP gene expression with IMF(intramuscular fat) and fatty acid content of longissimus muscle. Ten Laiwu pigs and seven Duroc weighting approximately 100 kg were chosen for the experiment. Expression of H-FABP at mRNA in LM of Laiwu pigs and Duroc were measured by real time fluorescence RT-PCR，I MF and fatty acid content were measured. The result indicated that，Compared with Duroc，H-FABP gene expression of longissimus muscle in Laiwu pigs was increased by 36. 16% . Correlation between H-FABP gene expression and IMF content with SFA(saturated fatty acid) ，SFA + MUFA (monounsaturated fatty acids) in Laiwu pigs Duroc was prominent. Correlation between H-FABP gene expression and IMF content with PUSA(polyunsaturated fatty acid) was distinctly negative，with sum of fatty acid in Laiwu pigs was not remarkable，t hat's of Duroc was contrary. Correlation between H-FABP gene expression with IMF content and fatty acid composing was marked，so H-FABP is candidate gene of IMF in Laiwu pigs＇ breeding.

Key words: Laiwu pigs, Real-time fluorescence RT-PCR, H-FABP gene expression, IMF, Fatty acid

中图分类号: 

• Q78