摘要： 棉蚜是转Bt基因抗虫棉(简称Bt棉)上的非目标害虫,也是Bt棉上危害较严重的害虫之一。用酶标仪在室内测定了取食Bt棉GK12不同世代棉蚜中肠消化酶系的活力和比活力,包括蛋白酶、蔗糖酶、海藻糖酶和淀粉酶,并与取食亲本常规棉泗棉3号的棉蚜比较,以研究Bt棉对棉蚜中肠消化酶的短期影响和长期效应。结果发现,取食Bt棉1代、2代、3代和60代以上的棉蚜,与取食常规棉棉蚜的这4种消化酶的活力和比活力之间均无显著差异,在Bt棉上取食不同世代棉蚜的消化酶活力也无显著差异。表明无论从短期影响还是长期效应来看,Bt棉对棉蚜的中肠消化酶系均无显著影响。

关键词: 转Bt基因抗虫棉, 棉蚜, 消化酶

Abstract: Cotton aphid Aphis gossypii (Homoptera: Aphidae) is one of the seriously occurred non??target pests in transgenicBt cotton fields. The activit ies and specific activit ies of midgut digestive enzymes, including proteinase, amy?? lase, trehalase and sucrase, of cotton aphids fed on Bt cotton cv. GK12 for different generat ions were detected with microtiter plate reader, and were compared with those of cotton aphids fed on the non??Bt parental cotton cv. simian 3. Thus the short??term and long??term effects of Bt cotton on the digest ive enzymes of cotton aphids were determined. The re?? sults showed that there were no significant differences in the activities and specific activities of the four digest ive enzymes between the cotton aphids fed on Bt cotton and non??Bt parental cotton, no matter for one to three generations, or for over sixty generations. And there were no significant difference among the cotton aphids fed on Bt cotton for different generations. It indicated that Bt cotton had no significant impacts on the activities of cotton aphid??s midgut digestive enzymes both in the short run and long term.

Key words: Transgenic Bt insect??resistant cotton, Cotton aphid, Digestive enzyme

中图分类号: 

• S435.62