干旱对不同铃重棉花不同区位果枝叶可溶性蛋白及脯氨酸含量的影响

doi:10.7668/hbnxb.2008.05.036

摘要/Abstract

摘要： 利用防雨旱棚，研究了水分胁迫下3个不同铃重基因型棉花不同部位内围果枝叶片中可溶性蛋白和脯氨酸含量的变化。结果表明：在两处理下，各品种不同部位棉叶中可溶性蛋白含量呈现出了不同的变化趋势，反映了棉花不同部位对水分胁迫适应能力的差异。干旱使各部位3个品种各铃期内围果枝叶可溶性蛋白含量降低，其中，下部果枝叶中大铃品种和小铃品种可溶性蛋白含量降低的幅度大于中铃品种，各品种的中部果枝叶中的可溶性蛋白含量变化比下部平缓，表明此部位果枝叶生理状态在棉铃发育过程中相对稳定。在水分胁迫下，各时期不同品种各部位果枝叶中脯氨酸含量均比对照增加；大铃品种各部位果枝叶在干旱胁迫下脯氨酸含量增加幅度小于中、小铃品种。不同部位果枝叶相比，干旱时脯氨酸含量增加幅度总体表现为：下部果枝叶＞中部果枝叶＞上部果枝叶。棉花品种存在着对干旱胁迫反应敏感程度不同的脯氨酸代谢调节机制，脯氨酸可以作为棉花抗旱性的参考性生理指标。

关键词: 棉花, 基因型, 水分胁迫, 可溶性蛋白, 脯氨酸

Abstract: The waterproof installations were carried out to deal with the content of soluble protein and free proline of different boll weight genotypes and inner positions in cotton under water stress.The results showed that the content of soluble protein at different positions appeared different trends for these genotypes under the two treatments,it illustrate that different position had different capacities of adaptation to drought stress.Under water stress,the soluble protein content which in each positions were decreased in these genotypes at the whole stage,the big-boll and small-boll varieties in lower branch leaves showed less accumulation of soluble protein content,the change of the content of soluble content in middle branch leaves was smaller than the lower one for each genotype which showed that the physiological characters of the branch leaves was more stable in the boll development.Under drought stress,the free proline content at each branch leaves showed more accumulation than the CK,the big-boll variety had more accumulation of the content of free proline in each poison.The free proline content in the lower branch leaves was the most,and then the middle ones,the upper ones was the least.Different varieties had different regulation mechanism in the condition of drought,so free proline can be considered as a physiological index nearly correlative to drought of cotton.

Key words: Cotton, Genotype, Water stress, Soluble protein, Proline

中图分类号:

S562.01

刘灵娣, 李存东, 高雪飞. 干旱对不同铃重棉花不同区位果枝叶可溶性蛋白及脯氨酸含量的影响[J]. 华北农学报, 2008, 23(5): 165-169. doi: 10.7668/hbnxb.2008.05.036.

LIU Ling-di, LI Cun-dong, GAO Xue-fei. Effects of Water Stress on Soluble Protein and Free Proline Content of Different Boll Weight Genotypes in Cotton[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(5): 165-169. doi: 10.7668/hbnxb.2008.05.036.

http://www.hbnxb.net/CN/Y2008/V23/I5/165

参考文献

[1] 陈善福,舒庆尧.植物耐干旱胁迫的生物学机理及其基因工程研究进展[J].植物学通报,1999,16(5):555-556.
[2] 南建福,刘恩科,王计平,等.苗期干旱和施肥对棉花生长发育的影响[J].棉花学报,2005,17(6):339-342.
[3] 李少昆,肖璐,黄文华,等.不同时期干旱胁迫对棉花生长和产量的影响Ⅱ棉花生长发育及生理特性的变化[J].石河子大学学报:自然科学版,1999,3(3):259-263.
[4] 肖俊夫,刘祖贵,孙景生,等.不同生育期干旱对棉花生长发育及产量的影响[J].灌溉排水,1999,18(1):23-27.
[5] 董合忠,李维江,李振怀,等.旱地与水浇地棉花生长发育特点比较研究[J].山东农业科学,1998(6):20-22.
[6] 程林海,张原根.干旱和复水对棉花叶片几种生理指标的影响[J].华北农学报,1995,10(4):82-85.
[7] 董合忠,李维江,唐薇,等.干旱和淹水对棉苗某些生理特性的影响[J].西北植物学报,2003,23(10):1695-1699.
[8] 马富裕,杨建荣,李蒙春,等.花铃期土壤含水量对棉花群体生理和产量影响研究[J].石河子大学学报:自然科学版,1998,2(2):93-98.
[9] Read S M,Northcote D H.Minimization of variation in the response to different protein of the Coomassic Blue G dye dinding assay for protein[J].Anal Biochem,1981,116:53-64.
[10] 邹琦.植物生理学实验指导[M].北京:中国农业出版社,2000:72-75.
[11] Longstreth D J.Nutrient influence on leaf photosynthesis effects on nitrogen phosphorus and potassium for Gossypium hirsutrumL[J].Plant Physiology,1980,65(3):541-543.
[12] 白景文,罗承德,李西,等.两种野生岩生植物的抗旱适应性研究[J].四川农业大学学报,2005,23(3):290-294.
[13] 康俊梅,杨青川,樊奋成.干旱对苜蓿叶片可溶性蛋白的影响[J].草地学报,2005,13(3):199-202.
[14] 王保莉,杨春,曲东.环境因素对小麦苗期SOD、MDA及可溶性蛋白的影响[J].西北农业大学学报,2000,28(6):72-75.
[15] 李德全,邹琦.土壤干旱下不同抗旱性小麦品种的渗透调节和渗透调节物质[J].植物生理学报,1992,18(1):37-44.
[16] 张明生,谢波,谈锋.甘薯可溶性蛋白、叶绿素及ATP含量变化与品种抗旱性关系的研究[J].中国农业科学,2003,36(1):13-16.
[17] Tang Z C.The accumulation of free proline and its role in water stressed Sorghum seeding[J].Act Phytophysl Sinica,1989,15(1):105-110.
[18] Chetti M b.Genotypic variation in osmoregulants and their relationship with bioproductivity and grain yield in wheat[J].Annals of Plant Physiology,1996,10(2):118-123.
[19] Sundaresan S.Effect of water deficit stress on proline accumulation in two varieties ocassva(Manihot esculenta crants)differing in their tolerance to drought[J].Indian Journal of Experimental Biology,1996,34(2):159-162.
[20] Christine G.Water deficit-induced changes in concentrations in proline and some other amino acids in the phloem sap of alfalfa[J].Plant Physiol,1996,111:109-113.
[21] Smirnoff N,Cumbes Q J.Hydroxyl radical scavenging activity of compatible solutes[J].Phytochem,1989,28(4):1057-1060.
[22] 斐英杰,郑家岭,瘐红.用于玉米品种抗旱性鉴定的生理生化指标[J].华北农学报,1992,7(1):31-35.
[23] Bouchereau A,Aziz A,Larher F,et al.Polyamines and environmental challenges:recent development[J].Plant Science,1999,140(2):103-125.
[24] 汪洪鹰,揭雨成,肖爱平,等.不同基因型苎麻叶片脯氨酸积累差异研究[J].中国苎麻,1999,21:27-29.
[25] 王朝云,揭雨成.水分胁迫对红麻生理特性和产量的影响[J].作物学报,1999,21(6):746-749.
[26] 韩永华,郑易之,李甜,等.高温、渗透双重胁迫对大豆某些生理反应具有累加效应的初报[J].大豆科学,2001,20(1):41-44.
[27] 张美云,钱吉,郑师章.渗透胁迫下野生大豆游离脯氨酸和可溶性糖的变化[J].复旦学报,2001,40(5):558-561.
[28] Hanson A D,Nelsen C F,Everson E H.Evaluation of free proline accumulation as an index of drought using to contracting barely cultivars[J].Crop Sci,1977,17:720-726.
[29] 王邦锡,黄久常,王辉.不同植物在水分胁迫条件下脯氨酸的累积与抗旱性的关系[J].植物生理学报,1989,15(1):46-51.
[30] 高浦新.玉米在水分胁迫条件下脯氨酸的积累与抗旱性的研究探讨[J].江西农业学报,1997,9(4):73-76.
[31] Singh T N,Aspinall D,Paley L G.Proline accumulation and varietal adaptability to drought in barley:A potential metabolie measure of drought resistance[J].Nature New Biol,1972,236:188-190.
[32] 张成军,解恒才,郭佳秋,等.干旱对4种木本植物幼苗脯氨酸含量的影响[J].南京林业大学学报:自然科学版,2005,29(5):34-36.
[33] 王启明,徐心诚,吴诗光,等.干旱胁迫对不同大豆品种苗期叶片渗透调节物质含量和细胞膜透性的影响[J].种子,2005,24(8):9-12.
[34] 揭雨成,黄丕生,李宗道,等.苎麻抗旱性基因型差异及其生理生化机理研究[J].中国麻作,1998,20(1):1-6.
[35] 张明生,杜建厂,谢波,等.水分胁迫下甘薯叶片渗透调节物质含量与品种抗旱性的关系[J].南京农业大学学报,2004,27(4):123-125.
[36] 罗音,孙明高.干旱胁迫对5树种叶片中脯氨酸含量的影响[J].山东林业科技,1999(4):1-3.

选择文件类型/文献管理软件名称

选择包含的内容