摘要： 枯草芽孢杆菌NCD-2菌株对棉花黄萎病病原菌大丽轮枝菌具有较强的抗生作用。通过对NCD-2菌株培养条件的研究,结果表明在30℃、培养液初始pH为7.0和培养时间为2 d条件下,利用NB培养基培养该菌株所得培养液抑菌活性优于其他培养基,其培养液经硫酸铵沉淀所得的蛋白粗提液经RNA酶处理后的抑菌活性与对照相比差异不显著,经蛋白酶K和胰蛋白酶处理后的抑菌活性与对照相比差异显著;蛋白粗提液经60℃处理后的抑菌活性与对照相比差异不显著,经80,100,120℃处理后抑菌活性与对照相比差异显著,但经120℃处理30 min后仍有一部分抑菌活性,研究结果说明该菌株产生的抗菌物质存在性质上的差异。
关键词:
枯草芽孢杆菌,
抗菌蛋白,
大丽轮枝菌,
棉花黄萎病
Abstract: Bacillus subtilis NCD22 was isolated from cotton rhizosphere and showed significantly control effect on cot2ton verticillium wilt. Characteristics of the crude antifungal protein produced by strain NCD22 was evaluated under differ2ent culture conditions. The result showed that the strain could produce more antifungal substance under the optimum con2dition(cultured in NB broth for 2d under 30 ℃ and pH 710). Precipitatesof the fermentation of strain NCD22 was collectedby salt out with ammonium sulfate and showed strong inhibition effect against Verticillium dahliae. The culture of the am2monium sulfate precipitation from the crude protein extracts by RNase treatment,the antifungal activity compared withcontrol was no significant difference,when treated with Proteinase K,Trypsin,the antifungal activity compared with thecontrol was significantly. The activity of the antifungal substrates were investigated under different temperatures. The anti2fungal activity of the substrate at 60 ℃was no significant difference when it compared with control,while the antifungalactivity decreased at 80,100 and 120 ℃and showed significant difference compared with the control. However,the crudeprotein extracts still showed the antifungal activity after heat treatmentsfor 30 min under 120 ℃. Results indicated that theantifungal extracts of Bacillus subtilis NC-2 were several substrates with different traits.
Key words:
Bacillus subtilis,
Antifungal protein,
Verticillium dahliae,
Cotton verticillium wilt
中图分类号:
孟立花, 李社增, 郭庆港, 马平, 刘大群. 枯草芽孢杆菌NCD-2菌株抗菌蛋白初步分析[J]. 华北农学报, 2008, 23(1): 189-193. doi: 10.7668/hbnxb.2008.01.042.
MENG Li-hua, LI She-zeng, GUO Qing-gang, MA Ping, LIU Da-qun. Primary Analysis on the Antifungal Activity of the Protein Produced by Bacillus subtilis NCD-2 against Verticillium dahliae[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(1): 189-193. doi: 10.7668/hbnxb.2008.01.042.