外源NO对低温胁迫下小麦幼苗果聚糖合成及FBEs表达的影响

doi:10.3969/j.issn.1000-7091.2013.02.002

摘要/Abstract

摘要： 采用水培的方法,研究4℃低温胁迫0,24,48,96 h后,外源NO供体硝普钠(SNP)对小麦幼苗生长、可溶性总糖含量、可溶性糖各组分含量及果聚糖合成酶(FBEs)等的影响,并采用qRT-PCR方法测定小麦幼叶中FBEs基因mRNA水平的变化。结果表明,外源NO促进小麦幼苗生长,提高低温胁迫下小麦幼叶果糖、葡萄糖、低聚果糖(DP3)、可溶性总糖等含量和果聚糖:果聚糖-1-果糖基转移酶(FFT)活性,并且在mRNA水平上调节FBEs基因表达。外源NO能够通过调控FBEs基因表达,进而影响果聚糖合成酶活性,促进果聚糖积累,提高小麦抵抗低温的能力。

关键词: NO, 低温, 小麦, 果聚糖, FBEs表达

Abstract: The effects of exogenous NO on wheat(Triticum aestivum L.)growth,solubility sugar,fructan bio-synthesis metabolism etc. under 4℃ chilling stress were investigated in seedlings,subjected to different concentra-tions of sodium nitroprussiate(SNP,as nitric oxide donor) for 0,24,48,96 h. The results showed that,under chilling stress,exogenous NO could induce wheat weight,fructose,glucose,low DP fructan,total soluble sugar content and fructan:fructan 1-fructosyltransferase(FFT) activity. Moreover,NO could regulate thegene expression at this level in following ways:sucrose:sucrose 1-fructosyltransferase(1-SST) and sucrose:fructan 6-fructosyltransferase(6-SFT)gene expression were inhibited by high concentration of SNP while 1-FFT was induced. Our report owing that exogenous NO alleviated the negative effects of non-freezing low temperatures by regulating FEBs and accumulating fructans.

Key words: NO, Chilling stress, Wheat, Fructan, FBEs expression

中图分类号:

张黛静, 姜丽娜, 邵云, 李春喜, 李婷婷, 杨淑芳, 郭田. 外源NO对低温胁迫下小麦幼苗果聚糖合成及FBEs表达的影响[J]. 华北农学报, 2013, 28(2): 6-11. doi: 10.3969/j.issn.1000-7091.2013.02.002.

ZHANG Dai-jing, JIANG Li-na, SHAO Yun, LI Chun-xi, LI Ting-ting, YANG Shu-fang,gUO Tian. Effects of Exogenous Nitric Oxide on Fructan Anabolism and FBEs Expression in Wheat under Chilling Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(2): 6-11. doi: 10.3969/j.issn.1000-7091.2013.02.002.

http://www.hbnxb.net/CN/Y2013/V28/I2/6

参考文献

[1] 蔡剑,姜东.气候变化对中国冬小麦生产的影响
[J]. 农业环境科学学报,2011,3(9):1726-1733.
[2] 杨晓红,陈晓阳.果聚糖对植物抗逆性的影响及相应基因工程研究进展[J]. 华北农学报,2006,21(增刊): 6-11.
[3] Valluru R,Van den Ende W. Plant fructans in stress envi-ronments:emerging concepts and future prospects[J]. Journal of Experimental Botany,2008,59:2905-2916.
[4] Li H J,Yang A F,Zhang X C,et al. Improving freezing tolerance of transgenic tobacco expressing sucrose:sucrose 1-fructosyltransferasegene from Lactuca sativa[J]. Plant Cell Tissue and Organ Culture,2007,89:37-48.
[5] Ritsema T,Smeekens S. Engineering fructan metabolism in plants[J]. Journal of Plant Physiology,2003,160:811-820.
[6] Edelman J,Jefford Tg. The mechanism of fructosan me-tabolism in higher plants as exemplified in Helianthus tu-berosus[J]. New Phytologist,1968,67:517-531.
[7] Van den Ende W,Van Laere A. De-novo synthesis of fruc-tans from sucrose in vitro by a combination of two purified enzymes(sucrose:sucrose 1-fructosyltransferase and fruc-tan:fructan 1-fructosyltransferase) from chicory roots(Ci-chorium intybus L.) [J]. Planta,1996,200:335-342.
[8] Kawakami A,Yoshida M. Fructan: fructan 1-fructosyl-transferase,a key enzyme for biosynthesis ofgraminan ol-igomers in hardened wheat[J]. Planta,2005,223:90-104.
[9] Vijn I,Smeekens S. Fructan:more than a reserve carbohy-drate? [J]. Plant Physiology,1999,120:351-359.
[10] 高翔,佘茂云,殷桂香,等.小麦果聚糖合成基因6-SFT克隆和功能验证 [J]. 科技导报,2009,27(23):70-75.
[11] 张黛静,姜丽娜,邵云,等.NO 在小麦生长发育与环境胁迫响应中的作用[J].麦类作物学报,2009,29 (2):356-360.
[12] Manzer H S,Mohamed H. Al-Whaibi,Role of nitric ox-ide in tolerance of plants to abiotic stress[J]. Protoplas-ma,2011,248(3):447-455.
[13] Liu X W,Zhang X L,Zhang Y,et al. Cloning and ex-pression analysis ofglutathione reductasegene from cu-2 期张黛静等: 外源NO对低温胁迫下小麦幼苗果聚糖合成及FBEs表达的影响 11cumber(Cucumis sativus L.) treated by exogenous nitric oxide under low temperature stress[J]. African Journal of Biotechnology,2012,11(31):7819-7835.
[14] Andrea F P,Graciela L S,Horaciog P. Fructan metabo-lism in two species of Bromus subjected to chilling and water stress[J]. New Phytologist,1997,136(1):123-129.
[15] 李合生.植物生理生化实验原理和技术[M]. 北京: 高等教育出版社,2000:195-197.
[16] Cairns J,Winters A,Pollock C J. Fructan biosynthesis in excised leaves of Lolium temulentum L. Ⅲ. A comparison of the in vitro properties of fructosyl transferase activities with the characteristics of in vivo fructan accumulation
[J]. New Phytologist,1989,112:343-352.
[17] Housley T L,Volenec J J. Fructan content and synthesis in leaf tissues of Festuca arundinaceal[J]. Plant Physi-ology,1988,86:1247-1251.
[18] Wang C W,Van den Ende W,Tillberg J E. Fructan ac-cumulation induced by nitrogen deficiency in barley leaves correlates with the level of sucrose:fructan 6-fruc-tosyltransferase mRNA[J]. Planta,2000,211:701-707.
[19] Bohnert H J,Nelson D E,Jensen Rg. Adaptations to en-vironmental stresses[J]. The Plant Cell,1995,7:1099-1111.
[20] Couée I,Sulmon C,Gouesbetg,et al. Involvement of soluble sugars in reactive oxygen species balance and responses to oxidative stress in plants[J]. Journal of Experimental Botany,2006,57:449-459.
[21] Zhang Lg.,Zhou S,Xuan Y,et al. Protective effect of nitric oxide against oxidative damage in Arabidopsis leaves under ultraviolet-B irradiation[J]. Journal of Plant Biology,2009,52(2):135-140.
[22] 马向丽,魏小红,龙瑞军,等.外源一氧化氮提高一年生黑麦草抗冷性机制[J].生态学报,2005,25(6): 1269-1274.
[23] Dean J,Harper J. Nitric-oxide and nitrous-oxide produc-tion by soybean and winged bean during the invivo nitrate reductase assay[J]. Plant Physiology,1986,82:718-723.
[24] Wei J Z,Chatterton N J. Fructan biosynthesis and fructo-syltransferase evolution:the expression of the 6-SFT(su-crose: fructan 6-fructosyltransferase)gene in crested wheatgrass(Agropyron cristatum) [J]. Journal of Plant Physiology,2001,158:1203-1213.
[25] Van den Ende W,Mighiels A,Van Wonterghem D,et al. Cloning,developmental,and tissue-specific expression of sucrose:sucrose 1-fructosyl transferase from Taraxacum officinale. Fructan localization in roots[J]. Plant Physi-ology,2000,123:71-79.
[26] Neill S J,Desikan R,Clarke A. Hydrogen peroxide and nitric oxide as signalling molecules in plants[J]. Journal of Experimental Botany,2002,53:1237-1242.
[27] Mata Cg,Lamattina L. Nitric oxide induces stomatal closure and enhances the adaptive plant responses a-gainst drought stress[J]. Plant Physiology,2001,126 (3):1196-1204.

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