摘要： 为了确定啶虫脒的合理拌种剂量,有效防控小麦生育后期害虫危害,系统研究了小麦植株中啶虫脒残留的检测方法。研究确立了乙腈振荡提取、弗罗里硅土小柱净化小麦植株样本中啶虫脒残留的样本前处理方法,建立了色谱柱程序升温、NPD检测的气相色谱分析方法。检测结果显示,在优化色谱条件下,啶虫脒出峰时间为10.03 min,在0.1～200 mg/L质量浓度范围内线性响应良好,回归方程为Y=3 678.37X-3 595.04(R²=0.9998)。啶虫脒在小麦植株中3个添加水平的添加回收率范围为82.73%～119.53%,3次重复测定值的相对标准偏差介于1.31%～4.73%之间。仪器的最小检出量为0.74 ng,方法的最低检出限为0.018 mg/kg。试验表明,该方法简便、高效、经济、可靠,适合于小麦植株中啶虫脒残留量的测定。

关键词: 小麦植株, 农药残留, 啶虫脒, 气相色谱法, 检测

Abstract: In order to confirm the reasonable acetamiprid dose to control later wheat pests，the analysis method?of acetamiprid residue in wheat plants has been studied systematically. And a method for determination acetamiprid?residue in wheat plants have been developed by solid phase extraction(SPE) coupled with gas chromatography.?Wheat plant samples was firstly shacking extracted in acetonitrile，then purified and concentrated by florisil solid?phase extraction cartridge. The detection of acetamiprid was performed by GC-NPD using capillary program rising?temperature and NPD detector. Under the optimal conditions，the retention time of acetamiprid was 10. 01 minutes，?the calibration curves showed good linearity(Y = 3 678. 37X － 3 595. 04，R2= 0. 999 8)from 0. 1 mg/L to 200?mg/L concentration of acetamiprid，the recoveries at the three spiked concentration levels ranged from 82. 73% to?119. 53%，and the relative standard deviations(RSDs)for blank sample spiked with standard were in the range of?1. 31% to 4. 73%. The detection limit of acetamiprid was 0. 74 ng and the lowest concentration detected was 0. 018?mg/kg in wheat plants. The results indicated that the method is simple，efficient，economical and reliable，and it's?suitable for the determination of acetamiprid residues in wheat plants.

Key words: Wheat plants, Pesticide residues, Acetamiprid, Gas Chromatography, Determination

中图分类号: 

• S482.3