华北农学报 ›› 2012, Vol. 27 ›› Issue (2): 218-221. doi: 10.3969/j.issn.1000-7091.2012.02.041

所属专题: 水稻 生物技术

• 论文 • 上一篇    下一篇

分子标记选择水稻抗稻瘟病基因Pi40和Pib聚合体

王金明1, 林秀云1, 刘晓梅2, 孙强1, 李鹏志1, 张三元1   

  1. 1. 中国农业科技东北创新中心, 吉林长春 130033;
    2. 吉林省农业科学院植保所, 吉林公主岭 136100
  • 收稿日期:2011-12-27 出版日期:2012-04-28
  • 通讯作者: 张三元(1951-), 男, 上海人, 研究员, 主要从事水稻育种研究。
  • 作者简介:王金明(1978-), 男, 山西高平人, 副研究员, 博士, 主要从事水稻育种研究。
  • 基金资助:
    吉林省人事厅博士后项目(00237);吉林省农业科学院博士启动基金(00107);吉林省科技支撑计划(20106023)

Selecting the Pyramids of Blast Resistance Genes Pi40 and Pib in Rice Assisted by Molecular Marker

WANG Jin-ming1, LIN Xiu-yun1, LIU Xiao-mei2, SUN Qiang1, LI Peng-zhi1, ZHANG San-yuan1   

  1. 1. Notheast Agricultural Research Center of China, Changchun 130033, China;
    2. Plant Protection Institute, Jilin Academy of Agricultural Science, Gongzhuling 136100, China
  • Received:2011-12-27 Published:2012-04-28

摘要: 培育多个抗病基因聚合的水稻品种是提高其抗病广谱性和持久性的有效途径之一。利用水稻抗稻瘟病基因Pi40、Pib的特异PCR标记,对含有Pi40、Pib的水稻品系复合杂交F250个植株进行检测,从中选择到Pi40、Pib双基因聚合的抗病植株28个,为持久、广谱抗病水稻育种奠定了基础。

关键词: 水稻稻瘟病, Pi40, Pib, 聚合, 标记辅助选择

Abstract: Pyramiding resistance genes have been suggested as one of the most effective methods for proventing?breakdown of major gene conferring resistance to blast within a very short period. The PCR markers of the blast resistence gene Pi40 and Pib were used to identify the genes in F2plants of combination among the rice lines containing Pi40 and Pib respectively. The results indicated that all the plants containing any one gene,two genes of Pi40?and Pib showed resistane to blast,while the plants lacking the resistance gene were susceptible to blast. The tightlylinked markers of the resistance genes were powerful tools for the identification of Pi40 and Pib in breeding program. 28 plants pyramiding two genes of Pi40 and Pib were selected from 50 plants of the F2population. They can?be used as parents to breed rice for blast resistance.

Key words: Rice blast, Pi40, Pib, Pyramiding, Marker assisted selection

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引用本文

王金明, 林秀云, 刘晓梅, 孙强, 李鹏志, 张三元. 分子标记选择水稻抗稻瘟病基因Pi40和Pib聚合体[J]. 华北农学报, 2012, 27(2): 218-221. doi: 10.3969/j.issn.1000-7091.2012.02.041.

WANG Jin-ming, LIN Xiu-yun, LIU Xiao-mei, SUN Qiang, LI Peng-zhi, ZHANG San-yuan. Selecting the Pyramids of Blast Resistance Genes Pi40 and Pib in Rice Assisted by Molecular Marker[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(2): 218-221. doi: 10.3969/j.issn.1000-7091.2012.02.041.

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