摘要： 以田间患枣疯病树表型健康叶片、花变叶以及疯小叶为材料,健康树叶片为对照,测定了不同生长期各种叶片的叶绿素、可溶性蛋白、丙二醛(MDA)含量及3种保护酶活性,以期揭示植原体对枣树生理代谢的影响。结果表明,患病后叶片叶绿素、可溶性蛋白含量均显著降低,过氧化物酶(POD)、超氧化物歧化酶(SOD)活性及MDA含量升高,多酚氧化酶(PPO)活性差异不显著,不同患病程度的叶片中花变叶变化最显著。这些结果均表明枣疯病植原体侵染枣树后,树体的光合作用和保护酶系统受到明显影响。
关键词:
枣疯病,
叶绿素,
SOD,
POD,
MDA,
PPO,
可溶性蛋白
Abstract: The leaves of behaved healthy，leaves changed by flower and tiny leaves with jujube witches' broom?(JWB)of Ziziphus jujuba cv． Zanhuangdazao and the leaves of healthy tree as the control were used in this paper．?The content of chlorophyll，soluble protein，MDA and three kinds of protective enzymes activity of different leaves at?different growth stages were determined to reveal the effect of phytoplasma on jujube physiological metabolism． The?results showed that the content of chlorophyll and soluble protein of diseased leaves were significantly lower than?that of healthy leaves． The POD，SOD activity and MDA content of diseased leaves were significantly higher than?that of healthy leaves，PPO activity was not significantly changed，leaves changed by flower was the most significantly changed than the other diseased leaves． These results indicated that the photosynthesis and protective enzyme system of diseased trees were significantly affected by jujube witches' broom phytoplasma．
Key words:
Jujube witches' broom(JWB),
Chlorophyll content,
SOD,
POD,
PPO,
MDA,
Soluble protein
中图分类号:
刘雅倩, 彭龙, 宁强, 赵锦, 刘孟军. 枣疯病植原体对枣树叶绿素及几种保护性酶的影响[J]. 华北农学报, 2012, 27(2): 213-217. doi: 10.3969/j.issn.1000-7091.2012.02.040.
LIU Ya-qian, PENG Long, NING Qiang, ZHAO Jin, LIU Meng-jun. Effect of Jujube Witches' Broom(JWB) Phytoplasma on the Chlorophyll and Several Protective Enzymes in Jujube Trees[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(2): 213-217. doi: 10.3969/j.issn.1000-7091.2012.02.040.