摘要： 为了分析银蓝水貂血液蛋白多态性与其生长性状的关联性.利用不连续垂直板聚丙烯酰胺凝胶电泳对银蓝水貂血液中转铁蛋白(Tf)、抗胰蛋白酶(Pi-1、Pi-2)、维生素D结合蛋白(GC)3个蛋白位点进行检测,检测结果表明,GC没有多态性,Tf、Pi-1具有多态性(Pi-2分离效果不清晰),通过建立一般线性模型筛选银蓝水貂遗传标记,方差分析显示,Pi-1是对银蓝水貂体重影响较大的位点(P=0.013<0.05),Pi-1(P=0.029<0.05)和Tf(P=0.039<0.05)对银蓝水貂体长有显著影响,筛选出Pi-1 AA、Tf AC是银蓝水貂的高产基因型.为生化遗传标记辅助选择运用于银蓝水貂的选育提供了可靠的依据.

关键词: 银蓝水貂, 蛋白多态性, 经济性状, 标记辅助选择

Abstract: The aim of this study was to analyze the association of blood protein polymorphism with some economic traits in the sliver blue mink.Three blood protein loci of sliver blue mink breeds in China,which included protease inhibitor-1,protease inhibitor-2,Vitamin D binding protein(GC)and Transferrin(Tf) were detected by method of vertical polyacrylamide gelelectrophoresis.The results showed that GC loci in mink were monomorphic,Tf and Pi-1 loci were polymorphic,(Pi-2 was not detected clearly),General linear model was founded for the selection of biochemical genetic marker.Least squares variance analysis showed that there was significant difference for Pi-1 loci of sliver blue mink and weight(P=0.013<0.05),Pi-1(P=0.029<0.05)、Tf(P=0.039<0.05)loci of sliver blue mink and length.Pi-1 AA,Tf AC were the high yielding genetypes of sliver blue mink.For biochemical genetic marker assisted selection used in silver blue mink breeding to provide reliable basis.

Key words: Silver blue mink, Blood protein polymorphism, Economic character, Marker assisted selection

中图分类号: 

• S865.2+2