小麦蛋白质含量分子标记辅助选择的效果分析

doi:10.3321/j.issn:1000-7091.2007.02.010

摘要/Abstract

摘要： 对小麦回交后代的蛋白质含量进行了分子标记辅助选择研究。先对2个与蛋白质含量相关的Xgwm570和E41M62-168标记进行验证，表明其与蛋白质含量密切相关。再通过表型选择、分子标记辅助选择(MASBC₂)和不加选择(RNDBC₂)产生的BC₂中，蛋白质含量的平均值和群体中蛋白质含量高于16.5%的植株比例在PHEBC₂和MASBC₂之间没有显著差异，但均显著高于RNDBC₂。对群体中的植株按两分子标记的类型分为A(2个位点)、B(1个位点)和C(无)3大类，蛋白质含量的平均值和植株中蛋白质含量高于16.5%的比例从高到低的排列顺序为：A类植株>B类植株>C类植株。由此看来，利用分子标记辅助选择来培育高蛋白质含量小麦是十分有效的方法。

关键词: 小麦, 蛋白质含量, 分子标记辅助选择, 数量性状位点(QTL)

Abstract: Marker-assisted selection for protein content was conducted in backcross population in wheat. Experiments were designed first to confirm the linkage of molecular markers Xgwm570, E41M62-168 with protein content. Comparison with backcross population developed through phenotypic selection (PHEBC₂), by marker-assisted selection (MASBC₂) and by random mating where no selection has been applied (RNDBC₂), there was no significant difference between means of phenotypic and marker-assisted selection (16.2% ) for protein content and the percentage of plants which have protein content higher than 16.5%, but significantly higher than RNDBC₂ population. When the backcross-derived plants were divided into A (two loci), B (one locus) and C (null) according to their molecular marker types, their protein content values and the percentage of plants which have protein content higher than 16.5% ranked from high to low is type Atype Btype C. From these we could conclude that marker-assisted selection is an efficient method to breed protein content in wheat.

Key words: Wheat, Protein content, Marker-assisted selection, Quantitive trait locus

中图分类号:

S512.1+1.033

陈喜文, 陈德富, 李永君. 小麦蛋白质含量分子标记辅助选择的效果分析[J]. 华北农学报, 2007, 22(2): 39-42. doi: 10.3321/j.issn:1000-7091.2007.02.010.

CHEN Xi-wen, CHEN De-fu, LI Yong-jun. Effect of Marker-assisted Selection on Protein Content in Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(2): 39-42. doi: 10.3321/j.issn:1000-7091.2007.02.010.

http://www.hbnxb.net/CN/Y2007/V22/I2/39

参考文献

[1] Dong H, Cox T S, Sears R G, et al. High molecular weight glutenin genes: effect on quality in wheat[J]. Crop Sci, 1991, 31: 974-979.
[2] Bhatt G M, Derera N F. Genotype environment interactions for heritabilities and correlations among quality traits in wheat[J]. Euphytica, 1975, 24: 597-604.
[3] Diehl A L, Johnson V A, Mattern P J. Inheritance of protein and lysine in three wheat crosses[J]. Crop Sci, 1978, 17: 391-395.
[4] Perretant M R, CadalenT, Charmet G, et al. QTL analysis of bread-making quality in wheat using a doubled haploid population[J]. Theor Appl Genet, 2000, 100: 1167-1175.
[5] Kuspira J, Unrau J. Genetic analysis of certain characters in common wheat using whole chromosome substitution lines[J]. Can J Plant Sci, 1957, 37: 300-326.
[6] Morris R, Mattern P J, Schmidt J W, et al. Studies on protein, lysine and leaf rust resistance in the wheat cultivar "Atlas 66" using chromosome substitutions[M]// Ramanujan S. Proceedings of the Fifth International Wheat Genetic Symposium. New Delhi: Indian Agricultural Research Institute, 1978: 447-454.
[7] Stein I S, Sears R G, Gill B S, et al. Heterogeneity of the "Wichita" wheat monosomic set for grain quality and agronomic traits[J]. Crop Sci, 1992, 32: 581-584.
[8] 陈喜文, 刘晓光, 陈德富, 等. 影响小麦加工品质数量性状位点的研究[J]. 南开大学学报: 自然科学版, 2006, 39(6): 6-11.
[9] Saghai-Maroof M A, Soliman K M, Jorgenson R A, et al. Ribosomal DNA spacer-length polymorphisms in barley: mendelian inheritance, chromosomal location, and population dynamics[J]. Proc Nat Acad. Sci USA 1984, 81: 8014-8018.
[10] Roeder M S, Korzun V, Wendenhake K, et al. A microsatellite map of wheat[J]. Genetics, 1998, 149: 2007-2023.
[11] Vos P, Hogers R, Bleeker M, et al. AFLP: a new teehnique for DNA fingerprinting[J]. Nucleic Acids Research, 1995, 23: 4407-4414.
[12] Ribaut J, Beltran M. Single Large-scale marker-assisted selection[J]. Mol Breedins, 1999, 5: 531-541.
[13] Hasha C T, Bhasker A G, Raja, et al. Opportunities for marker-assisted selection (MAS) to improve the feed quality of crop residues in pearl millet and sorghum[J]. Field Crops Research, 2003, 84: 79-88.
[14] Ioannidoua D, Pinela C, Brugldoua L, et al. Characterisation of the effects of amajor QTL of the partial resistance to Rice yellow mottle virus using a near isogenic-line approach[J]. Physiological and Molecular Plant Pathology, 2003, 63: 213-221.
[15] Kellya J D, Geptsb P, Miklasc PN, et al. Tagging and mappins of genes and QTL and molecular marker-assisted selection for traits of economic importance in bean and cowpea[J]. Field Crops Research, 2003, 82: 135-154.
[16] Charles W, Polacco M, M Lynn Senior. Synergy of empirical breeding, marker-assisted selection, and genomics to increase crop yield potential[J]. Crop Science, 1999, 39: 1571-1583.

选择文件类型/文献管理软件名称

选择包含的内容