华北农学报 ›› 2006, Vol. 21 ›› Issue (3): 16-18. doi: 10.3321/j.issn:1000-7091.2006.03.005

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红掌叶片离体培养过程中内源激素含量的变化

夏时云1, 麦瑜玲2, 许继勇3, 刘焕新4, 郑添群2, 林书瀚2, 郑少平2   

  1. 1. 天津市农业生物技术研究中心, 天津 300384;
    2. 汕头市中蔬花卉有限公司, 广东汕头 515041;
    3. 汕头投资建设总公司, 广东汕头 515041;
    4. 天津市花卉产业中心, 天津 300112
  • 收稿日期:2005-11-30 出版日期:2006-06-28
  • 作者简介:夏时云(1963- ),男,四川金堂人,副研究员,主要从事植物细胞工程技术和观赏花卉植物组织培养技术研究工作。

Study on Endogenous Hormones Changes During Leaf in Vitro Culture of Anthurium andraeanum

XIA Shi-yun1, MAI Yu-ling2, XU Ji-yong3, LIU Huan-xin4, ZHENG Tian-qun2, LIN Shu-han2, ZHENG Shao-ping2   

  1. 1. Tianjin Agricultural Biotechnology Research Center, Tianjin 300384, China;
    2. Shantou Zhongshu Flower Company Ltd, Shantou 515041, China;
    3. Shantou Investment Constriction General Company, Shantou 515041, China;
    4. Tianjin Flower Industrial Center, Tianjin 300112, China
  • Received:2005-11-30 Published:2006-06-28

摘要: 应用酶联免疫吸附测定法(ELISA)研究了红掌叶片愈伤组织诱导和植株再生过程中内源激素的含量变化。结果表明:内源激素IAA,GA3,ABA,ZR在幼嫩叶片内的含量均较高;而在愈伤组织诱导阶段,4种内源激素均呈下降趋势;在植株再生过程中,内源IAA和GA3含量继续下降,ABA和ZR含量的变化因品种而异,粉冠军内源含量开始回升,亚历桑娜则继续下降至最低水平;在小苗移栽后,粉冠军的4种内源激素含量均急剧回升并保持较高水平,亚历桑娜中内源IAA和GA3含量继续下降至最低水平,而内源ABA和ZR含量回升保持较高水平。

关键词: 红掌, 离体培养, 内源激素

Abstract: Changes of endogenous hormones during the callus induction and buds differentiation in Anthurium andraeanum were studied by ELISA.The results showed that there was high content level of IAA,GA3,ABA,and ZR in leaflets,while they decreased during callus induction stage.The contents of IAA,GA3 kept decreasing,ABA,ZR of Ping Champion had increased slightly and that of Arizon were at low level respectively.After the plantlet transplanted,the four endogenous hormones in Pink Champion increased sharply while IAA,GA3 in Arizon still kept low level and ABA,ZR increased to a high level.

Key words: Anthurium andraeanum, In vitro culture, Endogenous hormones

中图分类号: 

引用本文

夏时云, 麦瑜玲, 许继勇, 刘焕新, 郑添群, 林书瀚, 郑少平. 红掌叶片离体培养过程中内源激素含量的变化[J]. 华北农学报, 2006, 21(3): 16-18. doi: 10.3321/j.issn:1000-7091.2006.03.005.

XIA Shi-yun, MAI Yu-ling, XU Ji-yong, LIU Huan-xin, ZHENG Tian-qun, LIN Shu-han, ZHENG Shao-ping. Study on Endogenous Hormones Changes During Leaf in Vitro Culture of Anthurium andraeanum[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(3): 16-18. doi: 10.3321/j.issn:1000-7091.2006.03.005.