摘要： 根据微生物间的拮抗作用，试从病地土壤中分离拮抗放线菌，探索利用放线菌对向日葵菌核病进行生物防治的途径。从内蒙古呼和浩特市、巴彦淖尔、包头等地所采集的土壤中分离出38个放线菌菌株，经过平板拮抗作用初测，有8个菌株（F-1、F-3、F-6、F-8、F-11、F-15、F-21、F-25）对向日葵核盘菌（Sclerotinia sclerotiorum）有拮抗作用。选出其中2个作用最明显的菌株（F-6、F-8），观察其在平板对峙培养中对向日葵核盘菌的抑制作用及其代谢产物对向日葵核盘菌的作用。结果表明：F-6、F-8菌株及其代谢产物对向日葵核盘菌均有明显的抑菌作用。同时，对F-6、F-8两菌株的培养性状和形态特征进行了初步观察。
关键词:
向日葵,
核盘菌,
拮抗放线菌,
代谢产物
Abstract: We explored the way of biological control by isolating antagonisticmycetes-fungus from pathogenic soil for Sclerotinia sclerotiorum.The result showed that 38 strains were isolated from soil samples derived from Huhhot,Bayannaoer and Baotou regions of Inner Mongolia.We observed that 8 strains,named as F-1,F-3,F-6,F-8,F-11,F-15,F-21,F-25,displayed antagonisms against S.sclerotiorum on the PDA plate.F-6 and F-8 were selected to study their inhibition to S.sclerotiorum growth and effects of their metabolic products on S.sclerotiorum of sunflower.Meanwhile,we examined the cultural and morphological characteristics of F-6 and F-8.Results showed that F-6,F-8 and their metabolic products could inhibit S.sclerotiorum growth on the PDA plate.
Key words:
Sunflower,
Sclerotinia sclerotiorum,
Antagonist icmycetesfungus,
Metabolic products
中图分类号:
胡俊, 刘正坪, 周洪友, 丁俊, 宋娟. 向日葵菌核病生防放线菌的分离筛选及拮抗作用的初探[J]. 华北农学报, 2006, 21(1): 96-99. doi: 10.3321/j.issn:1000-7091.2006.01.022.
HU Jun, LIU Zheng-ping, ZHOU Hong-you, DING Jun, SONG Juan. Studies on Isolation and Screening of Antagonisticmycetes-fungus and Its Antagonistic Effect for Sunflower Sclerotinia Rot[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(1): 96-99. doi: 10.3321/j.issn:1000-7091.2006.01.022.