摘要： 采用RAPD、RGA和SRAP分子标记技术对10个主要樱桃番茄品种进行了分子鉴别的研究。14个RAPD随机引物扩增后共产生了29个多态性位点；1对RGA引物产生了10个多态性位点；1对SRAP引物产生了5个多态性位点。分析单引物的品种鉴别能力发现，RGA和SRAP标记具有较高的鉴别效率。通过双引物组合分析发现，利用RAPD引物S311和RGA引物组PK1+PK2可以将10个樱桃番茄品种区分开来，获得了各品种独特的核酸指纹。另外，在标记位点较多的情况下聚类结果基本上可以反映各品种之间生物学性状的相似性及亲缘关系的远近，但当标记位点数过少情况下，在一定程度上将使聚类结果失去本意。
关键词:
樱桃番茄,
分子鉴别,
RAPD,
RGA,
SRAP
Abstract: Three kinds of molecular markers,including RAPD,RGA and SRAP were employed to differentiate 10 cherry tomato varieties.In this study,29 polymorphic loci were produced by 14 RAPD primers,10 polymorphic loci by one RGA primer pairs,and 5 polymorphic loci by one SRAP primer pairs.The informative loci by each primer or primer pair were used to identify 10 cherry tomato varieties,respectively.It showed that the RGA and SRAP marker were more effective.To distinguish the 10 varieties,two-primer-combinations were tested.Primer-combination S311 and PK1+PK2 can identify the 10 cherry tomato varieties effectively and produces specific fingerprint of each varieties.More over,cluster tree can indicate the real relationship between varieties when more polymorphic loci were employed,otherwise the result will be nonsense.
Key words:
Cherry tomato,
Molecular identification,
RAPD,
RGA,
SRAP
中图分类号:
于拴仓, 柴敏, 姜立纲. 主要樱桃番茄品种的分子鉴别[J]. 华北农学报, 2005, 20(5): 42-46. doi: 10.3321/j.issn:1000-7091.2005.05.010.
YU Shuan-cang, CHAI Min, JIANG Li-gang. Varieties Identification of Cherry Tomato Using Molecular Markers[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2005, 20(5): 42-46. doi: 10.3321/j.issn:1000-7091.2005.05.010.