利用SDS-PAGE鉴定转基因小麦HMW-GS的表达类型和后代遗传

doi:10.3321/j.issn:1000-7091.2005.02.001

摘要/Abstract

摘要： 采用SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)在蛋白质水平上对T₂～T₅代小麦转基因株系高分子量谷蛋白亚基(HMW-GS)的遗传表达及其分离进行了鉴定和分析.结果发现, 外源1Dx5和1Dy10基因在T₂代部分株系中表达;在有的株系中出现了原亚基(8亚基)的缺失或沉默和新亚基(暂定8*)的产生, 因而检测出2+5+10+12, 2+5+7+8*+10+12, 5+7+10, 2+7+10+12的多种类型.对2+5+10+12型株系进行了多代跟踪分析, 发现其在T₂～T₅代陆续发生分离, 存在于不同单株、不同单穗的子粒和同一单粒后代之间.经筛选, 获得了天然不存在的亚基类型为2+5+10+12, 2+10+12, 2+5+12等稳定表达的种质创新株系.

关键词: 转基因, 小麦, HMW-GS, SDS-PAGE, 分离

Abstract: The genotypes and inheritance of High-Molecular-Weight Glutenin Subunits (HMW-GS) in T₂～T₅ generations transgenic wheat plants were conduced by SDS-PAGE. The results showed that the exogenous 1Dx5 and 1Dy10 genes expressed in some plants in T₂ population, and in some plants caused some endogenous genes to be null and a new subunit to appear. Many genotypes such as 2+5+7+10+12, 2+5+7+8^*+10+12, 5+7+10, 2+7+10+12, therefore, were picked out. The lines with 2+5+10+12 continue to separate from T₂ to T₅ in offspring populations of different plants, different spikes form one plant or different grains form one spike. So we get some stably expressed novel wheat lines with 2+5+10+12, 2+10+12, 2+5+12, which do not exist in nature were obtained.

Key words: Transgenic, Wheat, HMW-GS, SDS-PAGE, Separation

中图分类号:

S512.1

杨凤萍, 梁荣奇, 陈绪清, 韩立新, 张晓东. 利用SDS-PAGE鉴定转基因小麦HMW-GS的表达类型和后代遗传[J]. 华北农学报, 2005, 20(2): 1-4. doi: 10.3321/j.issn:1000-7091.2005.02.001.

YANG Feng-ping, LIANG Rong-qi, CHEN Xu-qing, HAN Li-xin, ZHANG Xiao-dong. The Genotyes and Inheritance of High-Molecular-Weight Glutenin Subunits(HMW-GS) in Transgenic Wheat by SDS-PAGE[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2005, 20(2): 1-4. doi: 10.3321/j.issn:1000-7091.2005.02.001.

http://www.hbnxb.net/CN/Y2005/V20/I2/1

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