钙与钙调素对番茄果实乙烯生物合成和信号转导基因表达的调控

doi:10.3321/j.issn:1000-7091.2005.01.001

华北农学报 ›› 2005, Vol. 20 ›› Issue (1): 1-5. doi: 10.3321/j.issn:1000-7091.2005.01.001

所属专题： 番茄；生物技术；

• 论文 • 下一篇

钙与钙调素对番茄果实乙烯生物合成和信号转导基因表达的调控

王文雅^1,3, 吕静², 朱本忠¹, 常世敏⁴, 翟百强¹, 罗云波¹

1. 中国农业大学食品科学与营养工程学院, 北京100083;
2. 中国农业大学生物学院微生物系, 北京100094;
3. 北京化工大学生命学院, 北京100029;
4. 河北工程学院食品科学技术系, 河北邯郸057150

收稿日期:2004-08-16 出版日期:2005-02-28
作者简介:王文雅(1975-),河北保定人,博士,主要从事乙烯信号转导及果蔬采后生物技术研究工作。
基金资助:
国家重点基础研究规划(973)(G1999077701)项目资助

Effect of Calcium and Calmodulin on the Expression of Genes in Ethylene Biosynthesis and Ethylene Signal Transduction

WANG Wen-ya^1,3, LÜ Jing², ZHU Ben-zhong¹, CHANG Shi-min⁴, ZHAI Bai-qiang¹, LUO Yun-bo¹

1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing100083, China;
2. State Key Laboratory for Agricultural Biotechnology, China Agricultural University, Beijing 100094, China;
3. College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China;
4. Department of Food Science, Hebei Institute of Technology, Handan 057150, China)

Received:2004-08-16 Published:2005-02-28

摘要/Abstract

摘要： 为了研究CaM和Ca²⁺在调控乙烯生物合成和信号转导组分基因表达中的相互关系,利用272 mmol/L CaCl₂及272 mmol/L CaCl₂+100 µmol/L W5(或W7)处理绿熟期番茄果实圆片,然后采用无菌培养的方法在20℃的条件下进行培养。结果表明:CaCl₂处理能够抑制番茄果实圆片的乙烯生成,抑制LeACO1,NR,LeERF2基因的表达, 而CaCl₂+W5(或W7)能消除Ca²⁺对果实圆片乙烯生成LeACO1,NR,LeERF2基因的表达的抑制作用,但CaCl₂和 CaCl₂+W5(或W7)处理均对LeACS2基因表达影响不明显。以上研究结果表明外源钙处理对乙烯生物合成和信号转导途径中相关基因表达的影响可能与CaM有关。

关键词: 钙调素, 钙, 乙烯, 基因表达

Abstract: In order to address the role that calmodulin plays in the regulation mechanism of calcium on the genes expression of ethylene biosynthesis and signal transduction pathway, the studies were carried out. Discs excised from tomato fruit(Lycopersicon esculentum cv. Lichun)at mature green stage were treated with 272 mmol/L CaCl₂ or 272 mmol/L CaCl₂+100 µmol/L WS/W7 solution,and then cultured under the aseptic conditions at 20℃.The results showed that both ethylene production and expression of LeAC01,NR, LeERF2 genes could be inhibited by CaC1₂ treatment,and CaCI₂+WS/W7 treatment could prevent the inhibitory effect of CaCI₂ treatment on ethylene production and expression of LeAC01, NR,LeERF2 genes. However, either CaC1₂ or CaC1₂+WS/W7 treatment has little effect on the expression of LeACS2 gene. These indicated that the inhibitory effect of CaC1₂ on expression of genes in ethylene biosynthesis and ethylene signal transduction pathway might be related to calmodulin.

Key words: Calmodulin, Calcium, Ethylene, Gene expression

中图分类号:

S641.2

王文雅, 吕静, 朱本忠, 常世敏, 翟百强, 罗云波. 钙与钙调素对番茄果实乙烯生物合成和信号转导基因表达的调控[J]. 华北农学报, 2005, 20(1): 1-5. doi: 10.3321/j.issn:1000-7091.2005.01.001.

WANG Wen-ya, LÜ Jing, ZHU Ben-zhong, CHANG Shi-min, ZHAI Bai-qiang, LUO Yun-bo. Effect of Calcium and Calmodulin on the Expression of Genes in Ethylene Biosynthesis and Ethylene Signal Transduction[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2005, 20(1): 1-5. doi: 10.3321/j.issn:1000-7091.2005.01.001.

http://www.hbnxb.net/CN/Y2005/V20/I1/1

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