摘要： 利用105条S系列和100条Operon随机引物,对小麦-簇毛麦代换系(6V/6A)、两个易位系(6VS/6AL,6VS/6DL)及亲本簇毛麦(VV)、硬粒小麦(AABB)、栽培小麦(AABBDD)的多态性进行了筛选分析.80.95%的S系列引物扩增出了结果,且条带较清晰;在100条OP系列引物中均扩增出结果,条带清晰可见.从205个随机引物中发现,只有1个引物OPW03在含有簇毛麦Ⅴ染色体的个材料中均扩增出1条约570 bp的谱带,而在栽培小麦和硬粒小麦中没有发现.因此,可以推测这个分子标记(OPW03-570)是位于簇毛麦Ⅴ染色体短臂上的.

关键词: 簇毛麦, Pm21基因, 小麦, 代换系, 易位系, 抗白粉病基因, RAPD标记

Abstract: This study screened and analyzed the polymorphism of a wheat-Haynaldia villosa substitution line (6V/6A) and two wheat-Haynaldia villosa translocation lines and parental Haynaldia villosa (VV) and Triticum durum by using 105 pieces of S-series random primers and 100 pieces of Operon random primers. 80.95 percent of S-series random primers amplified the polymorphism and its bands are clear. In all 100 pieces of Operon random primers amplified the polymorphism and its bands can be seen clearly. In 205 pieces of primers, only one primer OPW03 amplified the 570 by band in four materials contained V chromosome of Hay-naldia villosa，but this band don’t exist in common wheat and Triticum durum.Therefore, this molecular marker (OPW03一s}o ) maY locate in the short arm of Haynaldia villosa }s V chromosome.

Key words: Haynaldia villosa, Pm2lgene, Wheat, Substitution lines, Translocation lines, Powdery mildew resistance gene ( Pm ) , RAPD markers

中图分类号: 

• Q343.1+7