摘要： 利用根尖细胞染色体压片和叶片保卫细胞叶绿体压片方法对西葫芦胚囊再生植株进行了倍性鉴定,结果表明,单倍体、二倍体和四倍体植株叶片表皮保卫细胞叶绿体数的均值分别为(.15±0.37),(8.05±0.76)和(16.05±0.69),其比例约为1:2:,说明叶片保卫细胞叶绿体压片方法能有效用于倍性鉴定;单倍体、双单倍体和四倍体叶片表皮保卫细胞的长度分别为(20.31±3.38)μm、(30.80±2.19)μm和(1.78±1.03)μm,其比例约为1:1.5:2,在胚囊再生植株倍性鉴定时也可以参照.
关键词:
西葫芦,
未授粉胚珠,
再生植株,
倍性鉴定方法
Abstract: Chromosome counting and chloroplast counting in guard cells were used in the ploidy determina-tion of the regenerated plants from unpollinated ovules of cucubita pepo.The results indicated the guard cell chloroplast numbers in haploid，diploid and tetraploid were (4.1510.37)，(8.0510.76 ) and(16.OS士0.69) respectively;The ratio of them was approximately 1:2:4，which showed chloroplast counting was a reliable and practicable method in ploidy determination. In addition, the size of guard cell of haploid, diploid and tetraploid were ( 20.31士3.38 ) hem，(30.80土2.19)p.m, (41.78土1.03 ) p.m respectively，and the ratio of them was ap-proximately 1:1.5:2，which showed the method of counting the size of guard cell was also a practicable method in ploidy determination of regenerated plants that we can refer to.
Key words:
Cucurbita ,
Unpollinated ovules,
Regenerated plants,
Methods for ploidy determination
中图分类号:
郭永强, 王建设, 张慧玲, 文彩香, 李海真, 蔡民华. 西葫芦胚囊再生植株倍性鉴定方法研究[J]. 华北农学报, 2004, 19(3): 80-83. doi: 10.3321/j.issn:1000-7091.2004.03.020.
GUO Yong-qiang, WANG Jian-she, ZHANG Hui-ling, WEN Cai-xiang, LI Hai-zhen, CAI Min-hua. Study on Methods for Ploidy DetermiNa+tion in Regenerated Plant from UnpolliNa+ted Ovules of Cucurbita pepo[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2004, 19(3): 80-83. doi: 10.3321/j.issn:1000-7091.2004.03.020.