摘要： 研究了不同激素组合处理对鲁梅克斯K-1杂交酸模子叶愈伤组织的诱导,试验结果表明,2,4-D配合使用6-BAP时愈伤组织诱导率最高.在附加1.0 mg/L 2,4-D的液体MS培养基中通过悬浮培养分离出的单细胞,经数次继代后获得良好的悬浮培养体系.
关键词:
鲁梅克斯K-1杂交酸模,
子叶,
愈伤组织诱导,
悬浮培养
Abstract: This paper studied the effects of different hormone combinations and concentrations on callus induction hybrid sour dock cv.Rumex K-1.The experimental results showed that the induced rate of callus was the best by MS medium supplemented with 2,4-D and 6-BAP.The monocells were cultured in MS liquid medium supplemented with 1.0 mg/L 2,4-D was appropriate.After some times of culture,the cells suspension culture was set up
Key words:
Hybrid sour dock cv.Rumex K-1,
Coty ledon,
Callus induction,
Suspension culture
中图分类号:
杨起简, 周禾, 于同泉, 张蕾. 鲁梅克斯K-1杂交酸模愈伤组织诱导及细胞悬浮系的建立[J]. 华北农学报, 2002, 17(3): 75-79. doi: 10.3321/j.issn:1000-7091.2002.03.015.
YANG Qijian, ZHOU He, YU Tongquan, ZHANG Lei. Study on Callus Induction and Cell Suspension Line of Hybrid Sour Dock cv.Rumex K-1[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2002, 17(3): 75-79. doi: 10.3321/j.issn:1000-7091.2002.03.015.