摘要： 非编码区ITS（The Internal Transcribed Spacer）片段是目前分子系统学研究中应用最广的分子标记之一。运用生物信息学ORF finder技术，以单子叶网状叶脉植物的ITS片断为例进行分析，探讨了ITS内编码区5.8S和非编码区ITS1和ITS2的基本位置。并通过分析单子叶网状叶脉植物和菝葜科部分种类的ITS序列特点，研究了该片段在科、属、种间存在的关系和变异特点。
关键词:
ITS序列,
ORFfinder,
单子叶网状叶脉植物,
菝葜科
Abstract: ITS(the Internal Transcribed Spacer) is one of the most widely used molecular sequences in molecular systematics.Employing ORF finder of bioinformatics,ITS Sequences of monocots with reticulate vein were analysed,and posit the coding fragment 5.8S and ITS1 and ITS2.By analysing the feature of ITS sequence of monocots with reticulate vein,the differentiation and variation characters of ITS sequences in the families,genera and species studied were discussed.
Key words:
ITS Sequence,
ORF finder,
Reticulate vein monocots,
Smilacaceae
中图分类号:
司源, 郭亦琦, 孔航辉. 基于ORF Finder方法的植物ITS片段结构特点分析[J]. 华北农学报, 2005, 20(5): 54-56. doi: 10.3321/j.issn:1000-7091.2005.05.013.
SI Yuan, GUO Yi-qi, KONG Hang-hui. Analysis of Structure of ITS Sequence on Monocots with Reticulate Vein Basen ORF Finder[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2005, 20(5): 54-56. doi: 10.3321/j.issn:1000-7091.2005.05.013.