华北农学报 ›› 2011, Vol. 26 ›› Issue (6): 16-19. doi: 10.7668/hbnxb.2011.06.004

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核糖体蛋白L41与黄曲霉抗性的关系

严海燕1, 宗成志1, 马国华1, 单世华2   

  1. 1. 中南民族大学生命科学学院, 湖北武汉 430074;
    2. 山东省花生研究所, 山东青岛 266100
  • 收稿日期:2011-09-02 出版日期:2011-12-28
  • 作者简介:严海燕(1961-),女,湖南人,教授,博士,主要从事植物发育分子生物学研究。
  • 基金资助:
    山东省花生所合作项目(HZY05013);国家自然科学基金项目(NSFC307711361)

Relationship between Ribosomal Protein L41 and Resistance of Aspergillus flavus

YAN Hai-yan1, ZONG Cheng-zhi1, MA Guo-hua1, SHAN Shi-hua2   

  1. 1. College of Life Sciences, South-Central University for Nationalities, Wuhan 430074, China;
    2. Shandong Peanut Research Institute, Qingdao 266100, China
  • Received:2011-09-02 Published:2011-12-28

摘要: 已知与编码核糖体蛋白L41(RPL41)的CD399094相似的基因序列在黄曲霉敏感品种发育中种皮中上调表达。从花生黄曲霉抗性品种J11中克隆了相似基因。通过分析比较来自开放数据库中黄曲霉抗性和敏感品种花生发育中种子表达的RPL41序列,并对黄曲霉抗性和敏感品种果实不同发育时期和部位及叶片用荧光定量PCR做进一步的分析,研究在不同花生品种中核糖体蛋白L41与黄曲霉抗性的关系。结果表明,发育中种子表达的RPL41在抗性品种中多于敏感品种,而且多出的EST序列与在敏感品种中表达的不同,可能是特异地在抗性品种中表达。RPL41在抗性品种中发育中果实的果荚和子叶以及叶片上调表达,可能与该特定类型的RPL41est的专一表达以及与黄曲霉的抗性有关。

关键词: 花生, 黄曲霉, 核糖体蛋白L41:果实发育, 子叶

Abstract: Expression of CD399094-like gene encoding RPL41is up-regulated in developing seed coat of A. flavus sensitive cultivar. We have cloned similar RPL41est from resistant cultivar J11. The relation of RPL41in different peanut cultivars to A. flavuswas studied through comparison and analysis of similar ests from sensitive and resistant cultivars in GeneBank database,and quantitative PCR on different peanut fruit tissues from different developmental stages of A. flavusresistant and sensitive cultivars. The results showed that more RPL41were expressed in developing seeds of resistant varieties than that of sensitive cultivars, in addition, the specific type of RPL41est was expressed in A. flavusresistant strains. This indicates that higher expression level of ribosomal protein L41 in developing fruit shell,cotyledon and leave is possible related to specific expression of this specific RPL41ests and to Aspergillus flavus resistance.

Key words: Peanut, Aspergillus flavus, Ribosomal protein L41, Fruit development, Cotyledon

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引用本文

严海燕, 宗成志, 马国华, 单世华. 核糖体蛋白L41与黄曲霉抗性的关系[J]. 华北农学报, 2011, 26(6): 16-19. doi: 10.7668/hbnxb.2011.06.004.

YAN Hai-yan, ZONG Cheng-zhi, MA Guo-hua, SHAN Shi-hua. Relationship between Ribosomal Protein L41 and Resistance of Aspergillus flavus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(6): 16-19. doi: 10.7668/hbnxb.2011.06.004.

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