华北农学报 ›› 2001, Vol. 16 ›› Issue (3): 127-130. doi: 10.3321/j.issn:1000-7091.2001.03.025

所属专题: 黄瓜

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酵母多糖的制备及诱导黄瓜对灰霉病抗性的研究

岳东霞, 许长蔼, 张要武, 陈融, 崔汝玉, 郭俊焕   

  1. 天津市农业生物工程研究中心, 天津 300192
  • 收稿日期:2000-10-09 出版日期:2001-09-28
  • 作者简介:岳东霞(1969- ),女,助研,农学硕士,主要从事生物技术的研究工作。
  • 基金资助:
    国家科委“九五”攻关项目(99-010-01-10)

Preparation of Yeast Cell Walls β-(1→3)-glucan and Its Resistance Induction to Gray Mold of Cucumber

YUE Dongxia, XU Changai, ZHANG Yaowu, CHEN Rong, CUI Ruyu, GUO Junhuan   

  1. Tianjing Agricultural Biotechnology Research Centre, Tianjin 300192, China
  • Received:2000-10-09 Published:2001-09-28

摘要: 研究了用碱裂解法从Saccharomycescerevisiae中制备葡聚糖的方法,并通过红外光谱检测证明得到的产品主要是β-(1→3)-D-葡聚糖。葡聚糖作为诱导剂处理黄瓜种子,可使黄瓜植株产生对灰霉病的抗性,500mg/L和1000mg/L处理后1d的植株病叶率分别比对照下降了37.9~57.9和38~59个百分点,相对免疫效果分别为80.0%~90.0%和80.2%~92.1%;诱导处理1d后,植株病叶率升高,相对免疫效果下降。

关键词: &beta, -(1&rarr, 3)-D-葡聚糖, 诱导抗病性, 灰霉病

Abstract: The preparation of β (1→3) glucan by the alkalilysis method from Saccharomyces cerevisiae was studied in this paper.It was indicated that β (1→3) glucan was the major component of the acquired products by IR analysis.It could make the cucumber plants resistance to B.cinerea when used for seed soaking.The plants resistance could be maintained for 14 days.The rates of the diseased leaf of the plants induced with 500 mg/L and 1000 mg/L glucan were 37 9%-57.9% and 38.4%-59 0% lower than that of the control,respectively.The relative immunization efficiencies were 75 0%-90 9% and 77 5%-92.1%,respectively.

Key words: β-(1→3)-D-glucan, Induced resistance, Gray mold

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引用本文

岳东霞, 许长蔼, 张要武, 陈融, 崔汝玉, 郭俊焕. 酵母多糖的制备及诱导黄瓜对灰霉病抗性的研究[J]. 华北农学报, 2001, 16(3): 127-130. doi: 10.3321/j.issn:1000-7091.2001.03.025.

YUE Dongxia, XU Changai, ZHANG Yaowu, CHEN Rong, CUI Ruyu, GUO Junhuan. Preparation of Yeast Cell Walls β-(1→3)-glucan and Its Resistance Induction to Gray Mold of Cucumber[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2001, 16(3): 127-130. doi: 10.3321/j.issn:1000-7091.2001.03.025.

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