华北农学报 ›› 2001, Vol. 16 ›› Issue (3): 92-97. doi: 10.3321/j.issn:1000-7091.2001.03.018

所属专题: 萝卜

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高效液相色谱法测定蔬菜中的类胡萝卜素

宋曙辉, 薛颖   

  1. 国家蔬菜工程技术研究中心, 北京 100089
  • 收稿日期:2000-04-04 出版日期:2001-09-28
  • 作者简介:宋曙辉(1971- ),女,助理研究员,硕士,主要从事蔬菜营养方面的研究工作。

Determination of Carotinoids in Different Vegetables by High Performance Liquid Chromatography

SONG Shuhui, XUE Ying   

  1. National Engineering Research Center for Vegetables, Beijing 100089, China
  • Received:2000-04-04 Published:2001-09-28

摘要: 对4种类胡萝卜素的高效液相色谱(HPLC)测定方法进行比较,选出了适合本实验室的测定方法:分析柱为Nova-pakC18(3.9mm×300mm),以乙腈和三氯甲烷(92∶8)为流动相,检测波长为470nm和450nm双波长,并采用该方法对胡萝卜、南瓜、羽衣甘蓝3种蔬菜的数百份样品进行了α-胡萝卜素、β-胡萝卜素及茄红素含量的测定。

关键词: 高效液相色谱法, 蔬菜, 类胡萝卜素

Abstract: Four different methods were compared to determine the carotinoids with high performance liquid chromatography.The optimal method was selected,which adopted Nova pak C 18 (3.9 mm×300 mm)column and acetonitrile∶trichloromethane(92∶8) mobile phase.The contents of α carotene and β carotene in carrots,pumpkins and kales were determined by this method.

Key words: HPLC, Vegetable, Carot inoids

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引用本文

宋曙辉, 薛颖. 高效液相色谱法测定蔬菜中的类胡萝卜素[J]. 华北农学报, 2001, 16(3): 92-97. doi: 10.3321/j.issn:1000-7091.2001.03.018.

SONG Shuhui, XUE Ying. Determination of Carotinoids in Different Vegetables by High Performance Liquid Chromatography[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2001, 16(3): 92-97. doi: 10.3321/j.issn:1000-7091.2001.03.018.

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