摘要： 在提取我国12个省主产棉区26个菌系gDNA的基础上，采用OPY和OPB两组随机引物，对这些菌系的基因组进行了PCR扩增和聚类分析，40个引物中，12个引物扩增出满意的适合于分析的多态性谱带。聚类分析将26个菌系分为A、B两个群，Va菌系（Verticillium alboatrum）属于单独的A群，B群则包括了所有的大丽轮枝菌系（Verticillium dahliae）。B群又分为Ba（Cs、Yc）及Bb两个亚群。Bb亚群下面分3个组，Bb1（AV2、AV3及Ly）、Bb2（Sa、Ji、Ke及Yc）和Bb3。Bb3是最大的一个组，包括供试的3个大丽轮枝菌落叶型的3个菌系（VD-8、T9、V44）和其他大部分非落叶型的菌系。不同的单孢之间RAPD分析的结果（引物OPY06检测出单孢之间2·06kb的差异条带）从分子水平证实了单孢间变异的发生。对河南安阳4个菌株单独聚类分析则表现出致病性测定与RAPD分析有一定的相关性。
关键词:
棉花黄萎病菌,
gDNA提取,
RAPD分析,
致病力测定,
相关性
Abstract: DNA extracted by CTAB method has longer fragment, higher purity and better stability. So it is suitable for the DNA extraction of cotton Verticillium wilt pathogen. The RAPD analysis has been performed with 26 cotton Verticillium strains in 40 primers (OPY, OPB), 12 primers amplified polymorphic DNA patterns. By the cluster analysis, 26 strains are divided into 2 clusters (A, B). V. alboatrum belong to cluster A, cluster B comprises the other Verticillium strains (25 strains); cluster B can be divided into 2 subclusters including Ba and Bb, subcluster Bb followed by 3 groups: Bb1, Bb2, Bb3. The group Bb3 is the largest group, including defoliating strains (T9, VD-8, V44) and majority of Verticillium dahliae strains. The result of RAPD analysis of 4 Anyang strains showed the correlation of pathogenicity of cotton Verticillium wilt pathogen with RAPD analysis. RAPD analysis of different single spores also proved that the genomic variation may be occur among different single spores from one species.
Key words:
Cotton,
Verticillium wilt pathogen,
DNA extraction,
RAPD analysis
刘学堂, 郭金城, 张元恩, 宋晓轩, 孙君灵. 中国主产棉区黄萎病菌的RAPD分析[J]. 华北农学报, 1999, 14(1): 107-114. doi: 10.3321/j.issn:1000-7091.1999.01.022.
Liu Xuetang, Guo Jincheng. RAPD Analysis of Cotton Verticillium Wilt Pathogens[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1999, 14(1): 107-114. doi: 10.3321/j.issn:1000-7091.1999.01.022.