华北农学报 ›› 1996, Vol. 11 ›› Issue (3): 31-34. doi: 10.3321/j.issn:1000-7091.1996.03.006

所属专题: 小麦

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“中国春”小麦不同缺体的RAPD分析

何聪芬, 黄占景, 沈银柱, 刘植义, 柏峰, 马闻师, 张召铎   

  1. 河北师范大学生物系, 石家庄 050016
  • 收稿日期:1995-03-06 出版日期:1996-09-28

RAPD Analysis on Different Nullisomic Genomes of Wheat“Chinese Spring”

He Congfen, Huang Zhanjing, Shen Yinzhu, Liu Zhiyi, Bai Feng, Ma Wenshi, Zhang Zhaoduo   

  1. Hebei Normal University, Shijiazhuang 050016
  • Received:1995-03-06 Published:1996-09-28

摘要: 利用PCR-90A型DNA扩增仪,对7个中国春小麦缺体核DNA进行了扩增。结果显示,中国春小麦缺体核DNA的RAPD产物可分为二种类型:(1)所有供试材料均能扩增出的片段,代表了核DNA在进化上的保守性。有一个引物检测到这类片段。(2)全部供试材料间存在的差异片段,这类片段是用来基因定位的主要依据。另外,建立中国春小麦缺体系统的RAPD指纹图谱对研究小麦的系统进化也将有重要意义。

关键词: 小麦, 中国春缺体, 核DNA组, RAPD技术

Abstract: RAPD analysis on seven nullisomic genomes of wheat “Chinese Spring”was conducted using four primers with 10mer oligonucleotides(OPT 13,OPT 14,OPT 6 and OPT 7).The RAPD products revealed that the amplfied DNA bands fell into two categories:those could be amplified in all genomes,represented the DNA stability in evolution;and those differed in the studied genomes,were the important bands for genome identification.Results also suggested that RAPD“fingerprints”assay of nuclear DNA could be used as a tool in the study of systematic evolution of wheat.

Key words: Nullisomic wheat Chinese Spring, Nuclear genome, RAPD

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引用本文

何聪芬, 黄占景, 沈银柱, 刘植义, 柏峰, 马闻师, 张召铎. “中国春”小麦不同缺体的RAPD分析[J]. 华北农学报, 1996, 11(3): 31-34. doi: 10.3321/j.issn:1000-7091.1996.03.006.

He Congfen, Huang Zhanjing, Shen Yinzhu, Liu Zhiyi, Bai Feng, Ma Wenshi, Zhang Zhaoduo. RAPD Analysis on Different Nullisomic Genomes of Wheat“Chinese Spring”[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1996, 11(3): 31-34. doi: 10.3321/j.issn:1000-7091.1996.03.006.

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