华北农学报 ›› 1995, Vol. 10 ›› Issue (S1): 47-50. doi: 10.3321/j.issn:1000-7091.1995.z1.010

所属专题: 小麦

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适合黄淮冬麦区小麦花药培养的新型癸培养基

海燕, 和现昌, 王金兰, 周阳   

  1. 河南省农业科学院小麦研究所, 郑州, 450002
  • 收稿日期:1994-12-30 出版日期:1995-12-31

A New Medium Suited for Anther Culture of Wheat in Huang-Huai Winter Wheat Areas

Hai Yan, He Xianchang, Wang Jinlan, Zhou Yang   

  1. Wheat Research Institute ,Henan Academy of Agricultural Sciences,Zhengzhou
  • Received:1994-12-30 Published:1995-12-31

摘要: 根据黄淮冬麦区小麦生态类型及栽培品种的特点,从降低SO4--、NO3-入手,设计研制了癸培养基。采用L16(45)正交试验筛选出优良配方。经多年试验,癸培养基的愈伤组织平均诱导率分别较C17、W14、N6培养基高1.3、2.06、2.42个百分点;并对各种不同的基因型都有广泛的适应性,且有促进愈伤组织形成早的显著特点。

关键词: 小麦, 花药培养, 癸培养基, 诱导率

Abstract: Kui medium was developed, basically by reducing the concentration of SO4-- and NO3-,based on the biotypes and genotypes of wheat cultivars in Huang-Huai winter wheat areas.An appropriate composition of the medium was selected by orthogonal design L16(45).The frequencies of callus induction were increased by 1.3, 2.06 and 2.42 points in percentage on average respectively when kui medium was used, compared with C17, W14, and N6 media.The results showed that kui medium promoted the initiation of callus formation significantly and suited for various genotypes.

Key words: Wheat, Anther culture, Kui medium, Callus induction

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引用本文

海燕, 和现昌, 王金兰, 周阳. 适合黄淮冬麦区小麦花药培养的新型癸培养基[J]. 华北农学报, 1995, 10(S1): 47-50. doi: 10.3321/j.issn:1000-7091.1995.z1.010.

Hai Yan, He Xianchang, Wang Jinlan, Zhou Yang. A New Medium Suited for Anther Culture of Wheat in Huang-Huai Winter Wheat Areas[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1995, 10(S1): 47-50. doi: 10.3321/j.issn:1000-7091.1995.z1.010.

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