华北农学报 ›› 1990, Vol. 5 ›› Issue (3): 48-51. doi: 10.3321/j.issn:1000-7091.1990.03.010

• 论文 • 上一篇    下一篇

冬花椰菜的小孢子胚胎发生研究

杨清1, 曹鸣庆1, J.E.Chauvin2   

  1. 1. 北京市蔬菜研究中心, 北京 100081;
    2. 法国德艾米利欧雷顿植物试验站, 瑞尼斯
  • 出版日期:1990-09-28

Microspore Embryogenesis from Anther Culture of Winter Cau lif lower (Brassica oleracea L. ssp. botrytis)

Yang Qing1, Cao Mingqing1, J.E.Chauvin2   

  1. 1. Bei jing Vegetable Research Centre, Beijing;
    2. Station d’Amelioration des Plantes, I NRA, Rennes, France
  • Published:1990-09-28

摘要: 应用花药培养技术在四个冬花椰菜(Brassica oleracea L.ssp.botrytis)基因型中获得小孢子胚胎.培养基中加入硝酸银能明显促进胚胎发生.在含125mg/1硝酸银的培养基上胚产量最高.培养基中加入0.5g/1活性碳,能提高小孢子胚胎发生频率.另外在培养基中用琼脂糖代替琼脂,也能提高胚产量.

关键词: 冬花椰菜, 花药培养, 小孢子, 胚胎发生

Abstract: Microspore embrycgenesis was induced by performing anther culture on four winter cauliflower genotypes. The most important improvement of embry-ogenesis was obtained on the culture medium containing 125mg/l silver nitrate. The addition of active charcoal at a low concentration (0.5g/l) increased the frequency of microspore embryogenesis. The 5g/l concentration led to poor yields. The gelifiant agent seems to have a strong effect on cmbryogenesis, in that the medium solidified with agar led to limited embryogenesis for only one genotype, whereas the medium solidified with agarcse could induce embryogenesis for all genotypes.

Key words: Winter cauliflower (Brassica oleracea L.ssp. botrytis), Anther cucture, Microspore embryogenesis

引用本文

杨清, 曹鸣庆, J.E.Chauvin. 冬花椰菜的小孢子胚胎发生研究[J]. 华北农学报, 1990, 5(3): 48-51. doi: 10.3321/j.issn:1000-7091.1990.03.010.

Yang Qing, Cao Mingqing, J.E.Chauvin. Microspore Embryogenesis from Anther Culture of Winter Cau lif lower (Brassica oleracea L. ssp. botrytis)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1990, 5(3): 48-51. doi: 10.3321/j.issn:1000-7091.1990.03.010.

使用本文