The purpose of this study is to identify transcripts of porcine circovirus-like virus P1.In this work,the RNAs of porcine circovirus-like virus P1 synthesized in PK15 cells were characterized by Northern Blot analysis using the DIG-labeled riboprobes.The results showed two RNAs were detected.They included ORF3 RNA and ORF1 RNA.Meanwhile, ORF1 RNA had both more abundant and longer expression than those of ORF3 RNA.Northern Blot analysis indicated that at least two viral-specific RNAs were synthesized in P1.infected PK15 cells.
WEN Li-bin, WANG Feng-zhi, HE Kong-wang, NI Yan-xiu, ZHANG Xue-han, LI Bin, WANG Xiao-min, GUO Rong-li, ZHOU Jun-ming, YU Zheng-yu, MAO Ai-hua, LÜ Li-xin, XIE Jian-ping
. Transcriptional Analysis of Porcine Circovirus-like Virus P1 by Northern Blot[J]. Acta Agriculturae Boreali-Sinica, 2014
, 29(4)
: 160
-163
.
DOI: 10.7668/hbnxb.2014.04.028
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