In order to utilize heterosis more efficiently,select and maintain pairwise pure beet male sterile lines and maintainer lines.In this study,we used the unstable beet male sterile lines and their corresponding maintainer lines in bagging selection process as experimental materials.Special primers have been developed for the cytoplasm fertility molecular identification.The results showed that:the special primers can identify male sterile lines and maintainer materials effectively.The primers used in this study can provide more effective service to the sugar beet breeding.We also optimized the extractive method of mitochondrial DNA in sugar beet.And we directly extract the genome DNA for molecular identification.It can simplify the experimental pro cess and improve the efficiency.We identified 160 pairs of germplasms of unstable beet cytoplasmic male sterility through VNTR molecular markers.The 40 pairs of stable pure beet sterile lines and their corresponding maintainer lines were identified.The 40 pairs of stable pure beet are 31303(04)(1 pair),31327(28)(1 pair),31329(30)(1 pair),31333(34)(1 pair),31339(40)(1 pair),31341(42)(5 pairs),313343(44)(5 pairs),31345(46)(4 pairs),31355(56)(2 pairs),31361(62)(1 pair),31365(66)(1 pair),31367(68)(1 pair),31373(74)(1 pair),32315(16)(1 pair),32321(22)(4 pairs),32323(24)(1 pair),32327(28)(4 pairs),32331(32)(3 pairs),32341(42)(1 pair),32343(44)(1 pair).
WANG Liang
,
BAI Chen
,
LI Xiao-dong
,
ZHANG Hui-zhong
. VNTR Molecular Marker Technology in the Application of Beet Sterile Lines Breeding[J]. Acta Agriculturae Boreali-Sinica, 2014
, 29(4)
: 135
-139
.
DOI: 10.7668/hbnxb.2014.04.023
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