VNTR Molecular Marker Technology in the Application of Beet Sterile Lines Breeding

  • WANG Liang ,
  • BAI Chen ,
  • LI Xiao-dong ,
  • ZHANG Hui-zhong
Expand
  • 1. Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Huhhot 010031, China;
    2. Inner Mongolia Agricultural University, Huhhot 010019, China

Received date: 2014-04-16

  Online published: 2014-09-20

Abstract

In order to utilize heterosis more efficiently,select and maintain pairwise pure beet male sterile lines and maintainer lines.In this study,we used the unstable beet male sterile lines and their corresponding maintainer lines in bagging selection process as experimental materials.Special primers have been developed for the cytoplasm fertility molecular identification.The results showed that:the special primers can identify male sterile lines and maintainer materials effectively.The primers used in this study can provide more effective service to the sugar beet breeding.We also optimized the extractive method of mitochondrial DNA in sugar beet.And we directly extract the genome DNA for molecular identification.It can simplify the experimental pro cess and improve the efficiency.We identified 160 pairs of germplasms of unstable beet cytoplasmic male sterility through VNTR molecular markers.The 40 pairs of stable pure beet sterile lines and their corresponding maintainer lines were identified.The 40 pairs of stable pure beet are 31303(04)(1 pair),31327(28)(1 pair),31329(30)(1 pair),31333(34)(1 pair),31339(40)(1 pair),31341(42)(5 pairs),313343(44)(5 pairs),31345(46)(4 pairs),31355(56)(2 pairs),31361(62)(1 pair),31365(66)(1 pair),31367(68)(1 pair),31373(74)(1 pair),32315(16)(1 pair),32321(22)(4 pairs),32323(24)(1 pair),32327(28)(4 pairs),32331(32)(3 pairs),32341(42)(1 pair),32343(44)(1 pair).

Cite this article

WANG Liang , BAI Chen , LI Xiao-dong , ZHANG Hui-zhong . VNTR Molecular Marker Technology in the Application of Beet Sterile Lines Breeding[J]. Acta Agriculturae Boreali-Sinica, 2014 , 29(4) : 135 -139 . DOI: 10.7668/hbnxb.2014.04.023

References

[1] 王有昭.中国甜菜主要品系细胞质育性相关片段的分子差异[D].哈尔滨:哈尔滨工业大学,2009.
[2] 刘巧红,程大友,罗成飞,等.甜菜TR1位点多态性分析及细胞质育性鉴定[J].中国甜菜糖业,2013(1):18-20.
[3] 田自华,张子义,张剑峰,等.甜菜细胞质雄性不育系与其保持系线粒体DNA的RAPD分析[J].分子植物育种,2004,2(6):817-822.
[4] 杨克科.甜菜抗除草剂转基因的研究[D].哈尔滨:黑龙江大学,2012.
[5] 王有昭,罗成飞,程大友.甜菜细胞质雄性不育在分子水平的研究进展[J].中国甜菜糖业,2009(3):32-34.
[6] 格里克B R,汤普森J E.植物分子生物学及生物技术的实用方法[M].李汝刚,译.重庆:重庆出版社,1999.
[7] 顾红雅,陈章良.植物基因与分子操作[M].北京:北京大学出版社,1997.
[8] 戴维斯L G,狄伯纳M D.分子生物学基本实验方法[M].Amsterdam:Elservier Science Publishing Co.,Inc,1986.
[9] 史树德.甜菜细胞质雄性不育系与保持系线粒体和叶绿体DNA的RAPD分析[D].呼和浩特:内蒙古农业大学,2002.
[10] 陈星,汪琦,黄迎春,等.水稻颖果总RNA提取方法的研究[J].北京师范大学学报:自然科学版,2005,41(1):79-81.
[11] 程大友,徐德昌,鲁兆新,等.利用一年生甜菜快速选育保持系技术的研究[J].中国甜菜糖业,2003(4):1-5.
[12] 李晓东,白晨,张惠忠.甜菜抗丛根病单粒型雄性不育系N9849A、B的选育[J].内蒙古农业科技,2006(2):11-13.
[13] 程计华.油菜NSa雄性不育细胞质的分子鉴定及恢复系筛选[D].武汉:华中农业大学,2007.
[14] 张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(1):19-22,27.
[15] 杨永超.番茄亲本与F1的RAPD分析及相关性分析[D].雅安:四川农业大学,2007.
[16] 邓荟芬.黑芥子酶协助蛋白基因启动子370 bp序列功能的缺失分析[D].长沙:湖南农业大学,2007.
[17] 范锦.砂梨上苹果茎痘病毒的分离鉴定及其 cp 基因遗传转化烟草研究[D].武汉:华中农业大学,2007.
Outlines

/