In order to understand the codon characteristics of MyoG gene in chicken and select the appropriate exogenous expression system,online tools CUSP,CHIPS and codonW software were used to analyze MyoG gene. MyoG gene was compared to other genes of chicken,model animal genomes and MyoG gene of other species.Result showed that MyoG of chicken was bias toward the synonymous codons with G and C at the third codon position.GC content was greater than AT content in CDS region. MyoG gene and other thirty-one genes of chicken shared similar codon bias toward the synonymous codons with G and C at the third codon position.Codon bias comparison to other species showed that differences of codon bias between MyoG gene and Mus musculus was the minimum,indicating that Mus musculus could be used as exogenous gene expression host of MyoG gene.Codon bias comparison to the MyoG gene of other species indicated that MyoG gene expressed high in all the thirty-two species,including Jinghai yellow chicken.Almost all the thirty-two MyoG genes were bias toward to GC.Result of clustering based on RSCU value and phylogenetic tree based on CDS region showed that species with close genetic relationship tend to have similar codon bias.
ZHANG Tao
,
ZHANG Gen-xi
,
HAN Kun-peng
,
WANG Jin-yu
,
FAN Qing-can
,
LI Ting-ting
,
DUAN Lian
,
WANG Yong-juan
. Characterization of Codon Usage of MyoG Gene in Jinghai Yellow Chicken[J]. Acta Agriculturae Boreali-Sinica, 2014
, 29(4)
: 71
-79
.
DOI: 10.7668/hbnxb.2014.04.012
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