Ascorbate peroxidase(APX)is a key enzyme which eliminates oxygen free radicals and increases plant resistance in adverse circumstances.In order to study the function of ascrobate peroxidose in cold treated maize leves.The complete open reading frame of APX gene was cloned from cold-stressed(4℃)maize inbred line E28 using the method of reverse transcription PCR(RT-PCR).The primers were designed according to the published APX cDNA sequences.The sequence of APX from E28 was 753 bp,encoding a protein of 250 amino acid residues with a predicted molecular weight of 27.3 kDa and a theoretical pI of 5.56.APX were constructed into the expression vector pET-28a(+)with full-length ORF,then transferred into E.coli.After inducing by IPTG,the product proteins of APX were detected by SDS-PAGE and the proteins were 27.3 kDa as expected.Salt tolerance analysis showed that the recombinant exhibit strong tolerance to salt than the non-recombinant bacteria,and the critical concentration of NaCl is 0.6 mol/L.Our researches could lay the foundation for the study of plant tolerance in the future.
REN Ying
,
ZHAO Mei-ai
,
GUO Xin-mei
,
PEI Yu-he
,
SONG Xi-yun
. Cloning and Prokaryotic Expression Analysis of APX Gene from Maize[J]. Acta Agriculturae Boreali-Sinica, 2014
, 29(4)
: 49
-55
.
DOI: 10.7668/hbnxb.2014.04.009
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