The analysis of genetic diversity, cluster and network relationship were carried out on mtDNA D-loop sequence of 6 yak populations in Gansu province.The results showed that:The length variation of complete mtDNA D-loop sequence was from 891 to 895 base pairs;the (G+C) content was 38.80%, the (A+T) content was 61.20%, which showed that mtDNA D-loop region was rich in A and T.There were 60 variable sites were found in mtDNA D-loop sequence in 6 yak populations.The variations were mainly concentrated in the range of 200 to 400 base pairs, indicating that the region is highly variable region of yak mtDNA D-loop region.Forty one haplotypes were found in 240 yak Individuals, and the number of haplotype found in Maqu yak and Luqu yak populations were the most, which was 21, and that followed by Xiahe yak populations (18).The minimum number of haplotypes (11) was found in Tianzhu white yak.Haplotype diversity and average number of nucleotide differences (k) analysis showed that the most abundant genetic diversity of 3 yak populations in Gannan, but Tianzhu White Yak population was the lowest.Forty one haplotypes constructed phylogenetic trees and networks relationship analysis showed Gansu 6 yak populations may have two maternal origin.
CHENG Shu-ru
,
ZENG Yu-feng
,
WANG Xin-rong
,
ZHANG Li-ping
,
WU Jian-ping
. Genetic Diversity and Cluster Analysis of Six Yak Populations in Gansu Province Using Mitochondrial D-loop Sequence[J]. Acta Agriculturae Boreali-Sinica, 2014
, 29(3)
: 16
-21
.
DOI: 10.7668/hbnxb.2014.03.004
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