Wheat seedlings can get heat tolerance after exposed to heat acclimation.In order to study the molecular mechanism,heat killing times of leaf were measured and genes expression of heat-shock proteins and antioxidant enzymes were analyzed by qRT-PCR after subjected to heat acclimation at 34℃ for 48 h then 42℃ for 12 h using heat-tolerance cultivar Han 6172 and heat-susceptible cultivar Shixin 733.Heat tolerance of two wheat cultivars were increased to different degrees after heat acclimation at 34℃ and became marked after subjected to 42℃,Han 6172 showed more stronger heat tolerance. TaHsp70 and TaHsp16.9 genes expression could be up-regulated with different degrees in heat-tolerance cultivar after heat acclimation at 34℃ too.Based on this,the genes expression were up-regulated stronger in heat-tolerance cultivar than in heat-susceptible cultivar when subjected to 42℃.Genes expression of Ta(Cu/Zn)SOD, TaMnSOD, TaApx1, TaApx4 and TaApx5 all could be up-regulated with different degrees in heat-tolerance cultivar after heat acclimation at 34℃,and most of their expression level were higher in heat-tolerance cultivar than that in heat-susceptible cultivar.When subjected to 42℃,gene expression of Ta(Cu/Zn)SOD, TaMnSOD, TaApx1 and TaApx4 were up-regulated more stronger in heat-tolerance cultivar than that in heat-susceptible cultivar.Compared to the time point of peak,all gene expression were not latter than that of heat killing time.Those results showed that genes of Hsp and antioxidant enzyme participated in regulating the acquisition of heat tolerance during heat acclimation in wheat seedlings.The acquired heat tolerance were different in different wheat cultivars,the more stronger of acquired heat tolerance after heat acclimation,the longer it can be maintained,and the stronger heat tolerance crop showed after subjected to lethan temperature.
GUO Xiu-lin
,
LIU Zi-hui
,
ZHAO Hui-wei
,
XU Jing
,
LI Hui-cong
. Gene Expression of Heat-Shock Proteins and Antioxidant Enzymes Under Heat Acclimation and Heat Stress in Winter Wheat[J]. Acta Agriculturae Boreali-Sinica, 2014
, 29(4)
: 13
-18
.
DOI: 10.7668/hbnxb.2014.04.003
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