The Expression Pattern of Seven PcEXPA Genes of Chili and Whangkeumbae Pear during Fruit Postharvest Storage

  • YANG Shao-lan ,
  • ZHANG Xiao-fei ,
  • ZHANG Xin-fu ,
  • WANG Ran
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  • College of Horticulture, Qingdao Agricultural University, Qingdao 266109, China

Received date: 2014-03-11

  Online published: 2014-09-19

Abstract

Chili and Whangkeumbae pear were used to examine the fruit firmness, ethylene production rate, soluble pectin content and the expression patterns of seven PcEXPA genes in this paper.During the postharvest storage, the fruit firmness of both Chili and Whangkeumbae pear were decreased, while Chili fruit maintained higher fruit firmness than Whangkeumbae fruit.The soluble pectin content of Whangkeumbae pear was higher than that of Chili pear.The expression of seven PcEXPA genes showed that the expression of PcEXPA2 was consistent with the changes of fruit firmness and the ethylene production rate, and were down-regulated by 1-MCP treatment in the two varieties of pear. PcEXPA2 was closely related to the fruit softening of Chili and Whangkeumbae pear.

Cite this article

YANG Shao-lan , ZHANG Xiao-fei , ZHANG Xin-fu , WANG Ran . The Expression Pattern of Seven PcEXPA Genes of Chili and Whangkeumbae Pear during Fruit Postharvest Storage[J]. Acta Agriculturae Boreali-Sinica, 2014 , 29(3) : 153 -158 . DOI: 10.7668/hbnxb.2014.03.028

References

[1] McQueen-Mason S J,Durachko D M,Cosgrove D J.Two endogenous proteins that induce cell wall extension in plants [J].The Plant Cell,1992,4:1425-1433.
[2] Cosgrove D J.Expansive growth of plant cell walls [J].Plant Physiol Biochem,2000,38(1/2):109-124.
[3] Georgelis N,Nikolaidis N,Cosgrove D J.Biochemical analysis of expansin-like proteins from microbes [J].Carbohydrate Polymers,2014,100:17-23.
[4] Lee Y,Choi D,Kende H.Expansins:ever-expanding numbers and functions [J].Current Opinion Plantogy Biologgy,2001,4(6):527-532.
[5] Paull R E,Irikura B,Wu P F,et al.Fruit development,ripening and quality related genes in the papaya genome [J].Tropical Plant Biology,2008,1(3/4):246-277.
[6] Choi D,Cho H T,Lee Y.Expansins:expanding importance in plant growth and development [J].Physiologia Plant,2006,126(4):511-518.
[7] Sampedro J,Lee Y,Carey R E,et al.Use of genomic history to improve phylogeny and understanding of births and deaths in a gene family [J].Plant Journal,2005,44(3):409-419.
[8] Rose J K,Lee H H,Bennett A B.Expression of a divergent expansin gene is fruit-specific and ripening-regulated [J].Proc Natl Acad Sci USA,1997,94(11):5955-5960.
[9] Brummell D A,Harpster M H,Civello P M,et al.Modification of expansin protein abundance in tomato fruit alters softening and cell wall polymer metabolism [J].The Plant Cell,1999,11:2203-2216.
[10] Civello P M,Powell A L,Sabehat A,et al.An expansin gene expressed in ripening strawberry fruit [J].Plant Physiol,1999,121(4):1273-1279.
[11] Harrison E P,McQueen-Mason S J,Manning K.Expression of six expansin genes in relation to extension activity in developing strawberry fruit [J].J Exp Bot,2001,52(360):1437-1446.
[12] Hayama H,Shimada T,Haji T,et al.Molecular cloning of a ripening-related expansin cDNA in peach:evidence for no relationship between expansin accumulation and change in fruit firmness during storage [J].J Plant Physiol,2000,157(5):567-573.
[13] Hayama H,Ito A,Moriguchi T,et al.Identification of a new expansin gene closely associated with peach fruit softening [J].Postharvest Biol Technol,2003,291(1):1-10.
[14] Wakasa Y,Hatsuyama Y,Takahashi A,et al.Divergent expression of six expansin genes during apple fruit ontogeny [J].Europ J Hort Sci,2003,68:253-259.
[15] Wakasa Y,Kudo H,Ishikawa R,et al.Low expression of an endopolygalacturonase gene in apple fruit with long-term storage potential [J].Postharvest Biol Technol,2006,39(2):193-198.
[16] Costa F,Van de W E G,Stella S,et al.Map position and functional allelic diversity of Md-Exp7,a new putative expansin gene associated with fruit softening in apple(Malus×domestica Borkh.) and pear(Pyrus communis) [J].Tree Genet Genomes,2008,4:575-586.
[17] Trujillo D I,Mann H S,Tong C S.Examination of expansin genes as related to apple fruit crispness [J].Tree genetics and genome,2012,8(1):27-38.
[18] Ishimaru M,Smith D L,Gross K C,et al.Expression of three expansin genes during development and maturation of Kyoho grape berries [J].J Plant Physiol,2007,164(12):1675-82.
[19] Yong W,Lu W J,Li J G,et al.Differential expression of two expansin genes in developing fruit of cracking-susceptible and-resistant litchi cultivars [J].J American Soc Horticultural Science,2006,131(1):118-121.
[20] Hayama H,Shimada T,Fujii H,et al.Ethylene-regulation of fruit softening and softening-related genes in peach [J].J Exp Bot,2006,57(15):4071-4077.
[21] Hiwasa K,Rose J K,Nakano R,et al.Differential expression of seven alpha-expansin genes during growth and ripening of pear fruit [J].Physiologia Plantarum,2003,117(4):564-572.
[22] Fishman M L,Levaj B,Gillespie D,et al.Changes in the physico-chemical properties of peach fruit pectin during ontree ripening and storage [J].Journal of the American Society for Horticultural Science,1993,118:343-349.
[23] Livak K J,Schmittgen T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method [J].Methods,2001,25(4):402-408.
[24] Brummell D A,Harpster M H.Cell wall metabolism in fruit softening and quality and its manipulation in transgenic plants [J].Plant Mol Biol,2001,47(1/2):311-340.
[25] Hiwasa K,Kinugasa Y,Amano S,et al.Ethylene is required for both the initiation and progression of softening in pear(Pyrus communis L.) fruit [J].J Exp Bot,2003,54(383):771-779.
[26] Cosgrove D J.Cell wall loosening by expansins [J].Plant Physiol,1998,118(2):333-339.
[27] Yang S L,Xu C J,Zhang B,et al.Involvement of both subgroups A and B of expansin genes in kiwifruit ripening [J].HortScience,2007,42(2):315-319.
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