To select specific mAbs for VP1 protein of P1 virus, epitopes of it were predicted by bioinformatics software, and then mAbs against different epitopes were prepared by the method of SEALTM and applied to immunohistochemistry and Western Blot analysis, respectively, for screening sensitive and specific mAbs.Results showed that there were three positive mAbs(mAb1, mAb6 and mAb9) in immunohistochemical staining and one mAb(mAb1) showed strong hyacinthine in Western Blot.In a nutshell, specific mAbs were screened for VP1 protein of P1 virus, which will contribute to the detection of P1 and its vaccine development.
WANG Feng-zhi
,
WEN Li-bin
,
YAO Huo-chun
,
HE Kong-wang
. Preparation and Application of the Monoclonal Antibodies for VP1 Protein of Porcine Circovirus-like Virus P1[J]. Acta Agriculturae Boreali-Sinica, 2014
, 29(3)
: 101
-104
.
DOI: 10.7668/hbnxb.2014.03.019
[1] Segalés J,Allan G M,Domingo M.Porcine circovirus diseases[J].Anim Health Res Rev,2005,6(2):119-142.
[2] 杨汉春.猪免疫抑制性疾病的流行特点与控制对策[J].中国畜牧兽医,2004,31(5):41-43.
[3] Fenaux M,Halbur P G,Haqshenas G,et al.Cloned genomic DNA of type 2 porcine circovirus is infectious when injected directly into the liver and lymph nodes of pigs:characterization of clinical disease,virus distribution,and pathologic lesions[J].Journal of Virology,2002,76(2):541-551.
[4] Muhling J,Raye W S,Buddle J R,et al.Genetic characterisation of Australian strains of porcine circovirus types 1 and 2[J].Australian Veterinary Journal,2006,84(12):421-425,416.
[5] Raye W,Muhling J,Warfe L,et al.The detection of porcine circovirus in the Australian pig herd[J].Australian Veterinary Journal,2005,83(5):300-304.
[6] 温立斌,何孔旺,倪艳秀,等.类猪圆环病毒因子P1核酸链型和极性的研究[J].华北农学报,2012,27(3):120-122.
[7] 温立斌,何孔旺,杨汉春.P1 因子分子克隆的体外感染性分析[J].畜牧兽医学报,2008,39(7):941-944.
[8] 温立斌,何孔旺,杨汉春,等.类猪圆环病毒 2 型因子P1和 P2 诱导 PK-15 细胞凋亡的研究[J].华北农学报,2008,23(6):84-86.
[9] 温立斌,何孔旺,杨汉春.类猪圆环病毒 2 型因子 P1 与 P2 株全长基因组 DNA 分子克隆的构建[J].江苏农业学报,2007,23(6):579-582.
[10] 温立斌,何孔旺,杨汉春,等.TUNEL法检测类猪圆环病毒 2 型因子P1 诱导猪免疫器官细胞凋亡的研究[J].华北农学报,2008,23(Z2):88-91.
[11] Wen L,He K,Xiao Q,et al.A novel porcine circovirus-like agent P1 is associated with wasting syndromes in pigs[J].PloS One,2012,7(8):e41565.
[12] 温立斌,何孔旺,杨汉春,等.类猪圆环病毒因子P1 感染对猪红细胞的影响[J].内蒙古农业科技,2009(2):46-48.
[13] 温立斌,何孔旺,杨汉春,等.类圆环病毒因子 P1 感染对猪外周血 T 淋巴细胞含量和增殖活性的影响[J].华北农学报,2009,24(Z1):1-4.
[14] 温立斌,何孔旺,杨汉春,等.类圆环病毒因子 P1 感染对猪外周血单核细胞中细胞因子 mRNA 转录的影响[J].江苏农业学报,2011,27(5):1157-1159.
[15] 温立斌,何孔旺,杨汉春,等.类圆环病毒因子 P1 感染对猪外周血单个核细胞抗病毒蛋白 mRNA 转录的影响[J].华北农学报,2010,25(Z1):7-11.
[16] 温立斌,王小敏,周俊明,等.类猪圆环病毒因子 P1 在自然感染仔猪体内的组织分布[J].中国兽医学报,2013,33(1):1-3.
[17] Wen L,He K,Yang H,et al.Prevalence of porcine circovirus-like agent P1 in Jiangsu,China[J].Virol J,2011,8(8):543.
[18] 温立斌,何孔旺,杨汉春.一株类猪圆环病毒 2 型因子 P1 的全基因组序列测定与分析[J].中国农业科学,2010,43(2):411-416.
[19] 温立斌,何孔旺,杨汉春,等.类猪圆环病毒因子 P1 VP2 蛋白二级结构与 B 细胞表位预测[J].华北农学报,2009,24(5):45-49.
[20] 郝洪平.类圆环病毒P1因子ORF2蛋白的原核表达及其单克隆抗体的制备和鉴定[D].南京:南京农业大学,2011.