Gene Cloning of Porcine Fc Receptor Gamma Subunit and Its Prokaryotic Expression

  • ZHANG Zhi-yuan ,
  • ZHANG Ji-xi ,
  • XU Hong-yun ,
  • LIU Xiao-ping ,
  • LU Xiao-yan ,
  • DUAN Er-zhen ,
  • XIA Ping-an ,
  • CUI Bao-an
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  • College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China

Received date: 2011-10-12

  Online published: 2014-10-14

Abstract

mRNA encoding porcine FcR gamma subunit obtained from PAMs of 90-day piglets was transcribed into cDNA through RT-PCR. Recombinant plasmid pET-FcRγ was constructed successfully by inserting the cDNA sequence into pET-32a, and expressed as soluble protein with a molecular weight of about 29 kDa. Following purification, recombinant protein FcRγ-His was used to immune mouse, and polyclonal antibody against it was obtained. Western-blotting indicated that such polyclonal antibody could react specifically with FcRγ-His. Indirect ELISA shows that the titer of the polyclonal antibody is 1∶ 8000, revealing the good immunogenicity of FcRγ-His. All these results provide the foundation for further studying the structure and function of FcR gamma subunit.

Cite this article

ZHANG Zhi-yuan , ZHANG Ji-xi , XU Hong-yun , LIU Xiao-ping , LU Xiao-yan , DUAN Er-zhen , XIA Ping-an , CUI Bao-an . Gene Cloning of Porcine Fc Receptor Gamma Subunit and Its Prokaryotic Expression[J]. Acta Agriculturae Boreali-Sinica, 2012 , 27(1) : 35 -38 . DOI: 10.3969/j.issn.1000-7091.2012.01.007

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