Proteomic Analyses of the Early Pm21 Wheat Near Isogenic Lines and Their Parents Affected by Powdery Mildew

  • LI Da ,
  • LI Qiang ,
  • GUAN Xi-zhen ,
  • ZHANG Wei-dong ,
  • TIAN Ji-chun
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  • College of Agronomy, Shandong Agricultural University, State Key Laboratory of Crop Biology, Sub Center of Cereal Quality Control and Test, Taian 271018, China

Received date: 2011-11-07

  Online published: 2014-10-14

Abstract

The purpose of this project was to study inoculated by E09 in wheat leaves to confer durable and broad spectrum resistance to wheat powdery mildew through proteomic analysis. To carry out the cytological observations of Wheat(Triticum aestivum) cultivars Bainong 3217 and near-isogenic Bainong 3217/Pm21(BC7F6) plants were inoculated with E09 powdery mildew after 5 days. Total proteins were extracted by nonionic detergent, were separated by two-dimensional gel electrophoresis(2-DE) 3 replicates and analysed through MALDI-TOF-MS/MS mass spectrometry. Microscopic observation results showed that Bainong 3217 leaves cell surface folds after 12hr, and spores growing significantly inhibited on leave surface of near-isogenic Bainong 3217/Pm21(BC7F6) after 2 days inoculation. Besides, hypersensitive reaction was found at 4th day post inoculation. And 15 of up-regulated proteins were identified homologous to known proteins in various databases by MALDI-TOF-MS/MS and MASCOT analysis. The predicted functions of the 5 proteins were related to Chloroplasts photosynthesis(40%), oxygen releasing(13%), Metabolites synthesis(20%), resistance, transcription. One result of leaves deepening green may cause by Enzyme Rubisco and Glutamine synthetase leaf isozyme proteins increased. These proteins increased after about 3 or 4 hours infection. Enzyme Rubisco and Ascorbate peroxidase expressed were up-regulated to protect Calvin cycle in high concentration of H2O2. In addition, g lucan endo-1, 3-beta-D-glucosidase precursor induced were up-regulated to suppress further development of haustoria. The potassium channel protein may play an important role in early disease-resistance signal transduction in Pm21.

Cite this article

LI Da , LI Qiang , GUAN Xi-zhen , ZHANG Wei-dong , TIAN Ji-chun . Proteomic Analyses of the Early Pm21 Wheat Near Isogenic Lines and Their Parents Affected by Powdery Mildew[J]. Acta Agriculturae Boreali-Sinica, 2012 , 27(1) : 5 -11 . DOI: 10.3969/j.issn.1000-7091.2012.01.002

References

[1] 肖世和,庄巧生.中国小麦品种改良及系谱分析[M] 北京:中国农业出版社,2003:469-487.
[2] 李洪杰,王晓鸣,宋凤景,等.中国小麦品种对白粉病的抗性反应与抗病基因检测[J] 作物学报,2011,37(6):943-954
[3] 牛吉山.小麦抗白粉病遗传育种研究[M] 北京:中国农业科学技术出版社,2007:21-25.
[4] Qi L L,Chen P D,Liu D J,et al.Identification,mapping and application of polymorphic DNA associated with resistance gene Pm21 of wheat[J] Genome,1996,39:191-197.
[5] 齐莉莉,陈佩度,刘大钧,等.小麦白粉病新抗原--基因Pm21[J] 作物学报,1995,21(3):257-262.
[6] 刘金元,陶文静,刘大钧,等.小麦簇毛麦易位系6VS/6AL 中6VS 的遗传传递及其所携Pm21 基因的遗传稳定性分析[J] 植物学报,1999,41(10):1058-1060.
[7] 孙晓丽,周荣华,贾继增.白粉菌诱导的小麦叶片程序性细胞死亡[J] 扬州大学学报:农业与生命科学版,2006,27(2):21-25.
[8] 郭兴启,温孚江,朱汉城.烟草感染马铃薯Y 病毒(PVY) 后光合作用的变化规律[J] 浙江大学学报:农业与生命科学版,2000,26(1):75-78.
[9] Li Q,Chen X M,Li D,et al.Differences in Protein Expression and Ultrastructure between Two Wheat Near Isogenic Lines Affected by Powdery Mildew[J] Russian Journal of Plant Physiology,2011,58(4):686-695.
[10] 肖明刚.玉米疯顶病病原菌检测和病害防治技术研究[D] 北京:中国农业科学院,2004:20-21.
[11] Andrej S,Matthias W,Ole V,et al.Mass Spectrometric Sequencing of Proteins from Silver-Stained Polyacrylamide Gels[J] European Molecular Biology Laboratory(EMBL) Anal Chem,1996,68(5):850 858.
[12] Marco T,Kogel K H,Huckelhoven R.Superoxide and hydrogen peroxide play different roles in the nonhost interaction of barley and wheat with inappropriate formae speciales of Blumeria graminis[J] Mol Plant-Microbe Interact,2004,17:304-312
[13] Li A L,Wang M L,Zhou R H,et al.Comparative analysis of early H2O2 accumulation in compatible and incompatible wheat powdery mildew interactions[J] Plant Pathol,2005,54:308-316.
[14] Bienert G P,Mφller A L B,Kristiansen A,et al.Specific aquaporins facilitate the diffusion of hydrogen peroxide across membranes[J] The Journal of Biological Chemistry,2007,282(2):1183-1192.
[15] Cao A Z,Xing L P,Wang X Y,et al.Serine/threonine kinase gene Stpk-V,a key member of powdery mildew resistance gene Pm21,confers powdery mildew resistance in wheat[C]//Proceedings of the National Academy of Sciences of the United States of America(PNAS).2011,1016981108.
[16] Niu J S,Yu L,Ma Z Q.Molecular clonging and characterization of a Beclin-1 like gene in wheat[J] Journal of Plant Physiology and Molecular Biology,2002b,28:275-280.
[17] Oliveira I C,Brears T,Knight T J,et al.Overexpression of cytosolic glutamine synthetase.Relation to nitrogen,light,and photorespiration[J] Plant Physiol,2002,129:1170-1180.
[18] Tucker M R,Paech N A,Willemse M T,et al.Dynamics of callose deposition and beta-1,3-glucanase expression during reproductive events in sexual and apomictic Hieracium[J] Planta,2001,212(4):487-498.
[19] 于振,李倩,赵建叶,江帆,等.栽培小麦Brock 和京411 感染白粉菌后蛋白质组的变化[J] 作物学报,2009,35(11):2064-2072.
[20] Lesser M P.Oxidative stress in marine environments:biochemistry and physiological ecology[J] Annu Rev Physiol,2006,68:253-278.
[21] 孙卫红,王伟青,孟庆伟.植物抗坏血酸过氧化物酶的作用机制、酶学及分子特性[J] 植物生理学通讯,2005,41(2):143-147.
[22] Najami N,Janda T,Barriah W,et al.Ascorbate peroxidase gene family in tomato:its identification and characterization[J] Mol Genet Genomics,2008,279:171-182.
[23] Nakano Y,Asada K.Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts[J] Plant Cell Physiol,1981,22:867.
[24] 曹宛虹.作为叶绿体H2O2 分解系统关键酶的抗坏血酸过氧化物酶[J] 植物生理学通讯,1994,30(6):452-458.
[25] 李强,张卫东,田纪春.小麦抗白粉病基因Pm21 抗病差异的蛋白质组学研究[J] 中国农业科学,2009,42(8):2778-2783.
[26] Amrutha R N,Sekhar P N,Varshney R K,et al.Genomewide analysis and identification of genes related to potassium transporter families in rice(Oryza sativa L.)[J] Plant Science,2007,172:708-721.
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