The objectives of this study were to clone and analysis the coding region of KAP1.4 gene and to predict KAP1.4 protein structure in order to provide basic molecular data for the study of wool and cashmere traits. The result showed that the coding region of KAP1.4 gene in cashmere goat and sheep was 579, 549 bp long and espectively encoded 192 amino acids, 182 amino acids. Bioinformatic analysis showed that there was no significant difference in the basic physical and chemical characteristics of KAP1.4 protein of cashmere goat and sheep. They was a non-transmembrane protein and possessed the same length and the signal peptide cleavage point, having a protein kinase C phosphorylation site, three casein kinase Ⅱ phosphorylation sites and six N-terminal acylation sites. The protein secondary structure was constructed by β-sheet, β turn and random coil. Tertiary structure prediction showed that there were significant differences of three-dimensional structure in space between cashmere goat and sheep KAP1.4 protein. It would further affect the function and become an impact factor on the regulation of wool or cashmere traits.
GENG Rong-qing
,
CHEN Yu-lin
,
WANG Lan-ping
,
YANG Zhao-xia
,
JIANG Wei
,
LIU Min
,
CAO Chun-na
. cDNA Clonging and Structure Analysis on Encoding Protein of KAP1.4 Gene from Cashmere Goat and Sheep[J]. Acta Agriculturae Boreali-Sinica, 2012
, 27(1)
: 1
-4
.
DOI: 10.3969/j.issn.1000-7091.2012.01.001
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