As a secondary messenger in plant cell, Ca2+ plays an important role during regulating the plant to drought resistance under osmotic stress. The change of Cytosolic Ca2+ concentration directly reflects the cell change which from the extracellular stimulation. In this report,the change of Ca2+ fluorescence intensity and concentration of protoplast of Maize primary root tipinduced by ABA were studied by Confocal technology. The protoplast was firstly prepared and high vigor cell was used. Firstly,the Ca2+ concentrations were measured after treated with ABA with in10 min. The results showd that the Ca2+concentration increased with the treatment time delay. This indicated that Ca2+ participated in the ABA transduction process in primary root tip. For further proving the origin of cytosolic Ca2+ after treatments,EGTA,Vp and FTP were added to the ABA solutionin the experiment respectively. The results showed that both EGTA and Vp obviously suppressed Ca2+ concentration increasing which wereinduced by ABA in the root tip cell. This indicated that the cytosolic free Ca2+ induced by ABA came from extracellular mainly, also little came from the release of cytoplasmic calcium pool. As CaM inhibitor,TFP could change the Ca2+ fluorescence intensity and Ca2+ concentration when plant were subjected to osmotic stress,this seemed to show that CaM perhaps participated in the process of signal transduction of root system under osmotic stress too, but the mechanism is not known today.
ZHANG Feng-lian1
,
GUO Wei1
,
ZHANG Hong-mei2
,
CUI Qiu-hua2
,
LIU Zi-hui2
. Changes of Cytosolic Calcium Concentration of Maize Primary Root Tip Induced by ABA[J]. Acta Agriculturae Boreali-Sinica, 2010
, 25(1)
: 161
-164
.
DOI: 10.7668/hbnxb.2010.01.032
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