To study characters of chitinase from Beauveria bassiana HFW-05 highly toxic to Bemisia tabaci and Plutella xylostella,the chitinase gene HFWBbchit1 was firstly cloned from Beauveria bassiana HFW-05.According to the published sequences of chit1 genes(EU828354),a pair of primers was designed for full length DNA cloning of HFWBbchit1 gene by PCR.Subsequently,the amplified fragment of HFWBbchit1 gene was inserted into Escherichia coli cloning vector pMD19-T and sequenced.Sequence analysis revealed a DNA sequence with a length of 1 047 bp and encoded an open reading frame consisting of 348 amino acids of 70 kDa peptide.The analysis shows that the gene sequence exhibited 98% identity to that of Beauveria bassiana NCIM1216 chitinase(EU828354)and Beauveria bassiana Bb0062(AY145440).The deduced amino acid sequence exhibited 99% identity to that of Beauveria bassiana Bb0062 chitinase(AAN41259)respectively.
ZHEN Wei1,2,DU Li-xin2,CAO Wei-ping2,WANG Rong-yan2,SONG Jian2,WANG Jin-yao2,FENG Shu-liang2
. Cloning and Analysis of a Chitinase Gene from Beauveia bassiana HFW-05[J]. Acta Agriculturae Boreali-Sinica, 2010
, 25(1)
: 36
-39
.
DOI: 10.7668/hbnxb.2010.01.007
[1] Kramer K J,Muthukrishnana S.Insect chitinases:molecular biology and potential use as biopesticide[J].Insect Biochem Mol Biol,1997,27:887-900.
[2] Kuranda M J,Robbins P W.Chitinase is required for cell separation during growth of Saccharomyces cerevisiae[J].J Biol Chem,1991,266:19758-19767.
[3] Kramer K J,Corpuz L,Choi H K,et al.Sequence of a cDNA and expression of the gene encoding epidermal and gut chitinase of Manduca sexta[J].Insect Biochem Mol Biol,1993,23:691-701.
[4] Ding X F,Gopalakrishnana B,Johnson L B,et al.Insect resistant of transgenic tobacco expressing an insect chitinase gene[J].Transgenic Res,1998,7:77-84.
[5] 金洁,张作法,时连根,等.昆虫病原白僵菌的分子生物学研究进展[J].科技通报,2007,23(6):842-846.
[6] 林海萍,魏锦瑜,毛胜凤,等.球孢白僵菌蛋白酶、几丁质酶、脂肪酶活性与其毒力相关性[J].中国生物防治,2008,24(3):290-292.
[7] 曹伟平,王金耀,冯书亮,等.球孢白僵菌HFW-05的诱变筛选及其对烟粉虱若虫的毒力测定[J].中国生物防治,2007,23(2):133-137.
[8] Khemika Songjang,Tawee Donchai,Paranya Chaiyawat,et al.Cloning and expression of Chitinase gene isolated from insect pathogenic fungi,Beauveria bassiana in Escherichia coli[J].Chiang Mai J Sci,2006,33(3):347-355.
[9] 贾海丽,韩立强,杨国宇,等.CD147基因在家兔肠道的克隆与结构预测[J].华北农学报,2009,24(1):93-96.
[10] 刘永斌,荣威恒,王峰,等.蒙古羊BMP15全长DNA序列的克隆与分析[J].华北农学报,2009,24(1):55-59.
[11] Tronsmo A,Harman G E.Detection and quantifieation of N-acetyl-beta-D-glucosaminidase,chitobiosidase,and endoehitinase in solutions and on gels[J].Anal Bioehem,1993,208(1):74-79.
