In order to study changes of PLD and LOX activity under chilling injury temperature(2℃ ),after being treated with hot water for 30 min at 38,45 and 52℃,cucumbers were stored at 2℃for 15 d.Electrolyte leakage,MDA concent,LOX and PLD activity were determined.The result showed that hot water treatment resulted in lower cell membrane permeability,reduced MDA accumulation,maintained low level of PLD and LOX activity,and 45℃for 30 min was the optimum one among three hot water treatments.
WANG Guo-ze
,
SHA Na
,
YUN Yue-ying
,
LI Jun-fang
,
WANG Wen-long
,
FU Wen-ju
. Effects of Hot Water Treatment on LOX and PLD Activity in Cucumber[J]. Acta Agriculturae Boreali-Sinica, 2009
, 24(6)
: 100
-102
.
DOI: 10.7668/hbnxb.2009.06.020
[1] Parkin K L,Kuo S J.Chilling-induced lipid degredation in cucumber fruit[J].Plant Physiol,1989(90):1049-1056.
[2] Wang X.Plant Phospholipases[J].Plant Physiology,2001(52):211-231.
[3] Hero R G.Modulation of phospholipase D and lipoxygenase activities during chilling.Relation to chilling tolerance of maize seedlings[J].Plant Physiol Biochem,1998,36(3):213-224.
[4] Campos P S,Quartin V,Ramalho J C,et al.Electrolyte leakage and lipid degradation account for cold sensitivity in leaves of Coffea sp.plants[J].J Plant Physiol,2003,160:283-292.
[5] 赵世杰,许长成,皱琦,等.植物组织中丙二醛测定方法的改进[J].植物生理学通讯,1994,30(3):207-210.
[6] 黄勇,吴兴中,Ahmad Q T,等.用酶联比色法测定肝脏磷脂酰胆碱专一性磷脂酶D及其应用[J].上海医科大学学报,1997,24(5):343-346.
[7] Todd T F,Paliyath G,Thompson J E.Effect of chilling on the activities of lipid degrading enzymes in tomato fruit microsomal membranes[J].Plant Physiol Biochem,1992(30):517-522.
[8] Alonso A,Queiroz C S,Magalhaes A C.Chilling stress leads to increased cell membrane rigidity in roots of coffee (Coffea arabica L.) seedlings[J].Biochim Biophys Acta,1997,1323:75-84.
[9] Yapa P A J,Kawasaki T,Matsumoto H.Changes of some membrane-associated enzyme activities and degradation of membrane phospholipids in cucumber roots due to Ca2+ starvation[J].Plant Cell Physiol,1986,27:223-232.
[10] Luris S,Ronen R,Lipsker Z,et al.Effects of paclobutrazol and chilling temperatures on lipids,antioxidants and ATPase activity of plasma membrane isolated from green bell pepper fruits[J].Phsiologia Plantarum,1994,91:593-598.
[11] Mao L C,Pang H Q,Wang G Z,et al.Effects of phospholipase D and lipoxygenase on reaponse of cucumber fruits to chilling stress[J].Postharvest Biology and Technology,2007 (44):42-47.
[12] Mao L C,Wang G Z,Zhu C G,et al.Involvement of phospholipase D and lipoxygenase in reaponse to chilling stress in postharvest cucumber fruits[J].Plant Science,2007,172(2):400-405.
[13] Lee S H,Ahn S J,Im Y J,et al.Differential impact of low temperature on fatty acid unsaturation and lipoxygenase activity in figleaf gourd and cucumber roots[J].Biochem Biophysical Res Commu,2005,330:1194-1198.
[14] Woolf A B,Watkins C B,Bowen J H.Reducing external chilling injury in stored Hass′avocados with dry heat treatments[J].J Amer Soc Hort Sci,1995,120:1050-1056.