Genetic analysis on isozymes and RAPD of E.canadensis,E.sibiricus,F1、CoⅠ、C1Ⅰ、CoⅡ和C1Ⅱ were done.The results showed that the polymorphism of EST isozymes were much more than that of SOD during the four breeding periods.At tillering stage,the results of esterase isozymes also showed that true hybrid could be identificate,and zymogram characteristics of esterase isozymes tended to that of female parent E.canadensis.At other three breeding periods,zymogram characteristics of esterase isozymes tended to that of male parent and there were little differences.Differences of SOD isozyme only lied in expression level,and there were no specific band.Analysis on RAPD showed that genetic identity and genetic distance of E.canadensis and E.sibiricus were 0.7and 0.328 5.Their genetic relationship was most furthest among 7 materials.And genetic relationship between hybrids and parents showed that hybrids tended to female parent E.canadensis.But that was very far to male E.sibiricus.It was much appropriate that RAPD was used to analyze genetic relationship.
LI Jing-huan
,
YUN Jin-feng
,
LU Tian-chi
,
ZHOU Bi-liu
. Genetic Analysis on Isozymes and RAPD of E.canadensis,E.sibiricus and Their Hybrids[J]. Acta Agriculturae Boreali-Sinica, 2009
, 24(6)
: 38
-45
.
DOI: 10.7668/hbnxb.2009.06.008
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