Hebei Yali pear as the test materials,through the determination of fruit browning index and the vari- ation trend of LOX isozymes of pulp,slow and rapid cooling treatment were used for studying browning effect and LOX isozymes activity of different harvest maturity pulp.The results showed that a serious degree of browning was as follows: late harvest fruit > early fruit picking > middle fruit picking,rapid cooling > slow cooling.Therefore, the best storage methods was that mining maturity harvest pear(mid- September) using the slow cooling treatment. On the other hand,was determined with non- denatured PAGE.There were 9 LOX isozyme belts in pulp of Yali.And the rapid and slow cooling for different maturity of Yali LOX isozymes had a certain effect.The expression of LOX isozymes for rapid cooling was higher than that for slow cooling.The order of LOX isozymes expression for different maturity of Yali was late fruit > middle fruit > early fruit.This result was consistented with the determination of fruit browning index.It implicated that LOX enzyme isozymes had a close relationship with pears pulp browning.
LI Xiao-dan
,
SONG Fang-yuan
,
ZHU Wen-qiang
,
LIANG Li-ya
,
YAN Shi-jie
. Study on LOX Isozymes Changes of Yali Pear Pulp after Harvest and Its Relationship with Fruit Browning[J]. Acta Agriculturae Boreali-Sinica, 2013
, 28(4)
: 214
-217
.
DOI: 10.3969/j.issn.1000-7091.2013.04.039
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