Study on the Improvement of Immunogenicity of Myostatin with Hepatitis B Core Antigen

  • MA Yi ,
  • CHEN Xiao-qiang ,
  • WANG Wen-jie ,
  • LI Qing ,
  • GUAN Hong ,
  • AN Xiaorong ,
  • CHEN Yong-fu
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  • 1. Tianjin Institute of Animal Science and Veterinary, Tianjin 300112, China;
    2. Tianjin Agricultural University, Tianjin 300384, China;
    3. State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100094, China

Received date: 2009-04-22

  Online published: 2014-10-14

Abstract

Myostatin is a member of the TGF-βsuperfamily that functions as a negative regulator of skeletal muscle development in mammals.Targeting the myostatin pathway may be an effective strategy for increasing muscle growth.To improve immunogenicity of myostatin,the possibility of hepatitis B core antigen as molecular adjuvant was studied.First,myostatin C-domain was amplified by PCR and fused to the gene of hepatitis B core antigen at the position of N-terminal,C-terminal and internal respectively.Then three expression vector pET-30a-MSTN/HBcAg (N),pET-30a-MSTN/HBcAg(C) and pET-30a-MSTN/HBcAg(M)was constructed.The fused proteins were expressed in E.coli. and were purified by Ni2+ affinity chromatography.Then male Kunming white mice were immunized with single fused protein or recombinant C-domain.The indirect ELISA assay was used to detect the titer of antiserum against myostatin.The results shown:The immune effect of fused proteins was significantly better than that of recombinant C-domain(P<0.05);The immune effect of fused protein MSTN/HBcAg(M)was the best in three fused protein.These results proved that HBcAg could be used as molecular adjuvant to improve the immunogenicity of myostatin.

Cite this article

MA Yi , CHEN Xiao-qiang , WANG Wen-jie , LI Qing , GUAN Hong , AN Xiaorong , CHEN Yong-fu . Study on the Improvement of Immunogenicity of Myostatin with Hepatitis B Core Antigen[J]. Acta Agriculturae Boreali-Sinica, 2009 , 24(5) : 197 -200 . DOI: 10.7668/hbnxb.2009.05.041

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