By using directional cloning,a cDNA Library in the fruit2bearing shoot of Ziziphus jujuba Mill during the early stages of flower bud differentiation was constructed.In 1388 positive clones,557 cDNA inserts were obtained.469 cDNA inserts,length over 500 bp,were selected and sequenced,390 useful inserts were obtained at last.In these useful sequences,281 ESTs (among them there were 101 repetitive ESTs) were higher similarity with the known genes of CNBI,and 68 ESTs were unknown protein that its sequence had published,another 41 ESTs were unknown sequence (new gene).The known genes were classified by the classification way of arabidopsis thaliana genes,the result indicated that there were the basal metabolism gene 46 genes,the protein synthesis and transfer gene including 27 genes,the photosynthesis gene including 24 genes,the cytoarchitecture gene including 22 genes,the signal transduction gene including 19 genes,the auxesis gene including 19 genes,the resistance adversity gene including 11 genes,the flower development gene including 6 genes,the membrane traffic gene including 4 genes,and the metal transfer gene including 2 genes.The expression of some genes may be relation to the properties of jujube trees′s high resistance to the adverse environment,for example,cold resistance,drought resistance,barrenness tolerance and heavy metal tolerance.
MENG Yu-ping
,
CAO Qiu-fen
,
SUN Hai-feng
,
ZHOU Hui
,
ZHANG Chun-fen
. Construction and Partial ESTs Analysis of a cDNA Library for Fruit2bearing Shoot in Ziziphus jujuba[J]. Acta Agriculturae Boreali-Sinica, 2009
, 24(5)
: 102
-106
.
DOI: 10.7668/hbnxb.2009.05.022
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