SSR Marker Linked to Cucumber Trichomes Characteristic Related Gene

  • WANG Hui-zhe ,
  • LI Shu-ju ,
  • YANG Rui-huan ,
  • GUAN Wei
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  • Tianjin Kernel Cucumber Research Institute, Tianjin 300192, China

Received date: 2013-05-17

  Online published: 2014-10-14

Abstract

The glabrous mutant F80WM was found in the inbred line F80 and can be inherited stably.F2and BC1 population derived from F1 of F80 (trichomes female) and F80WM(glabrous male) was used as materials to study the inheritance and molecular marker associated to the trichomes and glabrous trait.The results indicated that the glabrous trait was controlled by a pair of recessive gene,and the character of trichomes was dominant to that of glabrous.The polymorphism between trichomes and glabrous parents of cucumber were studied using BSA method and SSR technology.1 193 SSR markers were screened,and a dominant marker SSR01647 was obtained. Sequen- cing of the fragment indicated that the lengths were 164 bp.The trichomes F2 plants possessed the 164 bp frag- ments,and the glabrous F2 plants were no fragments.The marker was closely linked to the cucumber trichomes trait- related gene,and the marker was confirmed to be 100% accurate by F2 individuals.The trait was a useful marker for hybridization and purity identification of F1 hybrids.

Cite this article

WANG Hui-zhe , LI Shu-ju , YANG Rui-huan , GUAN Wei . SSR Marker Linked to Cucumber Trichomes Characteristic Related Gene[J]. Acta Agriculturae Boreali-Sinica, 2013 , 28(4) : 79 -82 . DOI: 10.3969/j.issn.1000-7091.2013.04.015

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