Karyotyping and G- banding Analysis of Abaga Horse

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  • 1. Research Center for Animal Genetic Resources of Mongolia Plateau, College of Life Sciences, Inner Mongolia University, Huhhot 010021, China;
    2. Inner Mongolia Saikexing Reproductive Biotechnology Co, Ltd, Inner Mongolia, Huhhot 011517, China;
    3. Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Huhhot 010018, China

Received date: 2013-03-21

  Online published: 2014-10-14

Abstract

In this paper,chromosomal karyotype of Abaga horse was studied by using the fibroblast cells culture method and conventional chromosome analysis techniques.The karyotyping of Abaga horse has been analyzed by standard and G- banding technique.The results showed that the diploid chromosome number of Abaga horse was 2n = 64.The chromosome karyotypes of male and female horse were 64,XY and 64,XX.The chromosome 1-13 is metacentric-submetacentric autosome;chromosome 14-31 is acrocentric autosome.Chromosome X is the second longest submetacentric chromosome and Y is the smallest acrocentric chromosome.Moreover,each chromosome of Abaga horse has some unique patterns of its bands,and there are some differences with the reported Araba horse. This is the first report of G- banding karyotype of Abaga horse in China.The karyotype generated in this study will provide the fundamental information for equine cytogenetics and diagnosis of genetic disease in Arab horses.

Cite this article

WANG Ai-hong1,HAN Hong-mei1,LIU Yong-bin1,3,LI Yun-xia2,LI Xi-he1,2 . Karyotyping and G- banding Analysis of Abaga Horse[J]. Acta Agriculturae Boreali-Sinica, 2013 , 28(4) : 69 -74 . DOI: 10.3969/j.issn.1000-7091.2013.04.013

References

[] 芒 来,杨永平,萨仁苏和,等 阿巴嘎黑马 激情草 原上奔腾的良驹[J]. 内蒙古科技与经济,2011(19): 5-8.
[2] 雷初朝,韩增胜,陈 宏,等. 关中马的染色体核型分 析[J]. 西北农林科技大学学报: 自然科学版,2001,29 (4): 48.
[3] 李军祥. 岩羊染色体核型分析[J]. 甘肃畜牧兽医,1999(4): 13-14.
[4] 王墨清. 普氏野马染色体核型及多态性研究[J]. 甘肃 农业大学学报,1995,(6): 214-218.
[5] 刘祖洞. 江绍慧. 遗传学实验[M]. 北京: 高等教育出 版社,2000
[6] 张焱如,乌尼尔夫,白克力亚,等. 内蒙古乌审马染色 体核型研究[J]. 华北农学报,2005,20(1): 108-110.
[7] Li X,Zhou S G,Imreh M P,et al. Horse embryonic stem cell lines from the proliferation of inner cell mass cells [J]. Stem Cells Dev,2006,15(4): 523-531.
[8] Freshney R I. Animal cell culture: a practical approach [M]. Oxford: Oxford University Press,1992: 329.
[9] 张静南,刘永斌,张金吨,等. 阿拉伯马染色体 G 带核 型分析[J]. 华北农学报,2011,26(2): 101-106.
[10] 陈伟平,赵淑娟,庞有志,等. 伏牛白山羊染色体核型 分析[J]. 黑龙江畜牧兽医,2008,5(5): 13-15.
[11] Yang F,Fu B,O'Brien P C,et al. Karyotypic relation-ships of horses and zebras: results of cross-species chro-mosome painting[J]. Cytogenet Genome Res,2003,102 (1-4): 235-243.
[12] 刁 英. 染色体核型研究的方法及应用[J]. 渝西学 院学报: 自然科学版,2004,3(2): 55-58.
[13] 晁玉庆,国向东,赖双英,等. 蒙古马染色体核型初步 分析[J]. 内蒙古农牧学院学报,1992,13(1): 104-108.
[14] 张静南. 马 驴和骡成纤维细胞培养 核型及其 G 带 分析研究[D]. 内蒙古: 内蒙古大学,2011.
[15] 张巧灵,董常生,贺俊平,等. 羊驼染色体核型及其 G 分带的研究[J]. 遗传,2005,27(2): 221-226.
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