By the PCR technique,seed-specific promoter (napin) and glycerol-3-phosphate dehydrogenase (GPD1) were amplified from Brassica napus and Saccharomyces cerevisiae INVSc1 genomic DNA,and cloned into the pMD182T vector,respectively.The two fragments were inserted into the multiple cloning sites of the binary vector pCAMBIA1390 using the pBluescriptKS as the intermediary cloning vector.The resulted seed-specific expression vector was named as p1390NG.The vector was transformed into the Agrobacterium tumefaciens strains LBA4404,EHA105 and AGL1.It may provide an excellent genetic resources for genetic improvement of the seed oil content of energy plant.
LI Qun
,
WANG Xue-ying
,
LIU Ye
,
RUAN Cheng-jiang
. Construction of A Seed2specific Plant Expression Vector Targeting the GPD1 Gene[J]. Acta Agriculturae Boreali-Sinica, 2009
, 24(5)
: 31
-34
.
DOI: 10.7668/hbnxb.2009.05.007
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